癌症是全球最重要的公共卫生问题之一。尽管体外研究对生物医学研究做出了巨大贡献，但动物在研究疾病的生物病理学和诊断以及寻找新的预防和治疗策略方面至关重要。乳腺癌是目前全球最常见的癌症，占全球所有新发癌症年发病例数的12.5%。尽管大鼠化学诱导的乳腺癌模型被广泛应用于乳腺癌研究中，但在癌症诱导、样本收集和分析方面存在着缺乏规范化的问题。因此，为了使结果分析更加一致，为有意使用这一模型的研究人员提供实用指南具有重要意义。因此，在本综述中，我们基于我们在该领域的丰富经验，为研究人员提供了一个详细的，一步一步实施大鼠乳腺癌模型的指南，并以此来获得最佳结果、实验的最大通量以及方便研究间的比较。版权：©Silva等人。

Cancer is one of the most important public health problems worldwide. Despite the great contribution of in-vitro studies for biomedical research, animals are essential to study diseases' biopathology and diagnosis, and searching for new preventive and therapeutic strategies. Breast cancer is currently the most common cancer globally, accounting for 12.5% of all new annual cancer cases worldwide. Although the rat model of mammary cancer chemically-induced is widely used to study this disease, there is a lack of standardization in procedures for cancer induction, sample collection, and analysis. Therefore, it is important to provide a practical guide for researchers aiming to work with this model to make the analysis of results more uniform. Thus, in this review, we provide the researchers with a detailed step-by-step guide to implement a rat model of mammary cancer, based on our wide experience in this field, to obtain the best results, maximum throughput of each experiment, and easy comparison among researches.Copyright: © Silva, et al.