利用纳米复合材料开发先进的甲氨蝶呤（MTX）传输具有重要的临床应用价值。本研究利用了聚乙二醇-聚丙氧基-聚乙二醇（Poloxamer 188）胶束和甲氨蝶呤（MTX）负载的层状双金属氢氧化物（LDH-MTX），通过剥离重组的方法制备了具有良好分散性和高效细胞摄取能力的LDH-MTX-Poloxamer 188纳米复合材料，用于控制药物传递。 LDH-MTX-Poloxamer 188纳米复合物呈现球状形态，其平均流体动力学直径小于100 nm，比裸露的LDH-MTX复合物具有更好的分散状态。重要的是，LDH-MTX-Poloxamer 188纳米复合物能够实现显著的持续药物释放，并具有明显的pH依赖的响应性释放能力。此外，这些纳米复合材料还表现出长期和优异的体外抗肿瘤效果，与纯MTX或LDH-MTX相比，从细胞存活率方面可以看出。更有趣的是，与用于模拟MTX的纯FITC相比，用FITC标记的LDH纳米复合物通过细胞摄取被认为具有更好的细胞黏附能力。因此，LDH-MTX-Poloxamer 188纳米复合物可以作为一种新型先进的MTX传递纳米载体，在未来的治疗方面具备所需的特性。

Exploitation of advanced methotrexate (MTX) delivery with nanocomposites has important clinical application value. Poloxamer 188 micelle and layered double hydroxide loaded with MTX (LDH-MTX) by exfoliation reassembling were used to prepare LDH-MTX-poloxamer 188 nanocomposites with good dispersibility and efficient cellular uptake for controlled drug delivery. The LDH-MTX-poloxamer 188 nanocomposites with sphere-like morphology, of which the average hydrodynamic diameter was <100 nm, were shown to have better dispersion state than naked LDH-MTX. Importantly, the LDH-MTX-poloxamer 188 nanocomposites could achieve significant sustained drug release and have obvious pH dependent responsive release ability. In addition, these nanocomposites also exhibited long-term and excellent in vitro antitumor efficacy as opposed to pure MTX or LDH-MTX as evident from cell viability. More interestingly, compared to pure FITC used to simulate MTX, LDH nanocomposites labeled with FITC were considered to have better cell adhesion through cell uptake. Therefore, the studied nanocomposites of LDH-MTX-poloxamer 188 can be further used as a new advanced MTX delivery nanovehicles with desired properties in future therapeutic aspects.