铁死是一种抑制多种人类癌症的有前途的策略。快速准确地评估生物样品对铁死的相对敏感性将加快铁死靶向疗法的发展。我们先前证明了光化学活化膜脂过氧化（PALP）可以利用高功率激光诱导局部多不饱和脂肪酸酰（PUFA）脂质过氧化，有效地报告活细胞和组织中的铁死敏感性。在这里，我们描述了PALP分析的实验细节，包括组织切片的制备、荧光脂质过氧化报告物的制备、样品染色、激光源引起的脂质过氧化以及数据处理。我们设想对细胞和组织样品的相对铁死敏感性进行预测可能对基础研究和临床调查有用。© 2023. 作者，由Springer Science+Business Media公司独家许可，隶属于Springer Nature。

Ferroptosis is emerging as a promising strategy for suppressing multiple types of human cancers. Rapid and accurate assessment of the relative sensitivity to ferroptosis in biological samples will accelerate the development of ferroptosis-targeted therapies. We previously demonstrated that photochemical activation of membrane lipid peroxidation (PALP) that uses high-power lasers to induce localized polyunsaturated fatty acyl (PUFA)-lipid peroxidation can efficiently report ferroptosis sensitivity in live cells and tissues in situ. Here, we describe the experimental details for PALP analysis, including preparation of tissue sections, preparation of fluorescent lipid peroxidation reporter, sample staining, lipid peroxidation induced by laser source, and data processing. We envision predicting the relative sensitivity to ferroptosis of cellular and tissue samples is potentially useful for basic research and clinical investigations.© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.