铁死亡是一种独特的铁依赖性细胞死亡形式，由脂质过氧化和随后的细胞膜破裂引起，与其他类型的调控性细胞死亡有所区别。铁死亡与多种生物过程（如衰老、免疫和癌症）相关。另一方面，器官样本是三维的小型器官模型体系，可进行离体培养，已在组织发育和疾病建模、药物筛选和细胞治疗方面引起广泛关注。器官样本对于提高我们对人类疾病的理解具有巨大潜力，尤其是在寻找器官病理过程中铁死亡领域。此外，由于铁死亡具有抗肿瘤效应，癌症器官样本被用于研究分子机制和体外药物筛选。目前，肝脏器官样本的开发已经达到相对成熟的阶段。在这里，我们介绍了产生肝脏器官样本和肝癌器官样本的方案，以及检测器官样本中铁死亡的方法。© 2023作者，通过专属许可授予Springer Science+Business Media有限责任公司，属於Springer Nature。

Ferroptosis is a unique form of iron-dependent cell death induced by lipid peroxidation and subsequent plasma membrane rupture, which sets it apart from other types of regulated cell death. Ferroptosis has been linked to a diverse range of biological processes, such as aging, immunity, and cancer. Organoids, on the other hand, are three-dimensional (3D) miniaturized model systems of different organs in vitro cultures, which have gained widespread interest for modeling tissue development and disease, drug screening, and cell therapy. Organoids offer tremendous potential for improving our understanding of human diseases, particularly in the search for the field of ferroptosis in pathological processes of organs. Furthermore, cancer organoids are utilized to investigate molecular mechanisms and drug screening in vitro due to the anti-tumor effect of ferroptosis. Currently, the development of liver organoids has reached a relatively mature stage. Here, we present the protocols for the generation of liver organoids and liver cancer organoids, along with the methods for detecting ferroptosis in organoids.© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.