泛素-蛋白酶体系统（UPS）是一种高度保守的细胞机制，用于降解和回收真核细胞中的蛋白质。它涉及将特定靶蛋白质与泛素标记，使其被蛋白酶体标记为降解对象，而蛋白酶体则是一个大型的蛋白复合物，起着分子粉碎机的作用。UPS功能紊乱已被认为与多种疾病有关，包括癌症、神经退行性疾病和病毒感染。因此，针对UPS已成为许多疾病的治疗策略之一。铁死亡是一种依赖铁的细胞死亡过程，其调控受到多个水平的影响，包括蛋白质降解。在本章节中，我们通过西方博蛋白法、共免疫共沉淀法、体外泛素化酶活性测定和蛋白酶体活性测定等多种技术，介绍了在铁死亡中检测UPS介导的蛋白质降解的方法。© 2023. 作者（S），在Springer Science+Business Media, LLC的独占许可下，属于Springer Nature的一部分。

The ubiquitin-proteasome system (UPS) is a highly conserved cellular mechanism that degrades and recycles proteins in eukaryotic cells. It involves the tagging of specific target proteins with ubiquitin, a small regulatory protein, which marks them for degradation by the proteasome, a large protein complex that acts as a molecular shredder. Dysfunction of the UPS has been implicated in a wide range of diseases, including cancer, neurodegenerative disorders, and viral infections. Therefore, targeting the UPS has become an attractive therapeutic strategy for many diseases. Ferroptosis is an iron-dependent cell death process that is regulated by multiple levels, including protein degradation. In this chapter, we introduce the detection of UPS-mediated protein degradation in ferroptosis using several techniques such as western blotting, co-immunoprecipitation, in vitro ubiquitination assay, and proteasome assay.© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.