铁死是一种依赖于铁离子的调节性细胞死亡形式，其主要原因是铁离子的积累、脂质过氧化和细胞膜破裂。铁死的过程和功能可以通过多种方式在体外和体内进行监测。患者源异种移植（PDX）是一种临床前癌症模型，将人类癌组织移植到免疫缺陷小鼠或其他动物模型中，通常从进行手术或活检的患者中获得。它是了解癌症药物反应的强大工具，因为PDX模型保留了原始肿瘤的生长环境和异质性。通过分析PDX模型中的铁死，我们有可能对人类肿瘤发生的机制有更深入的了解。在本文中，我们总结了在PDX模型中分析铁死的几种试验方法。© 2023. 作者，独家授权于Springer Science+Business Media, LLC，Springer Nature的一部分。

Ferroptosis is a form of iron-dependent regulated cell death that is primarily caused by the accumulation of iron, lipid peroxidation, and subsequent rupture of the plasma membrane. The process and function of ferroptosis can be monitored in multiple ways, both in vitro and in vivo. Patient-derived xenograft (PDX) is a type of preclinical cancer model that involves transplanting human cancer tissue, usually obtained from patients undergoing surgery or biopsy, into immunodeficient mice or other animal models. It is a powerful tool for understanding drug response in cancer, as PDX models preserve the growth environment and heterogeneity of the original tumors. By analyzing ferroptosis in PDX models, we can potentially gain insights into human tumorigenesis. In this article, we summarize several assays used to analyze ferroptosis in PDX models.© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.