铁过氧化死亡是一种由氧化损伤驱动的调控性细胞死亡形式，其特点是铁超载和脂质过氧化，并由一系列独特的分子和细胞器调节。受损的铁过氧化反应已被证明涉及多种生理和病理过程，包括肿瘤发生、神经退行性疾病和缺血再灌注损伤。免疫组织化学（IHC）和免疫荧光（IF）是研究铁过氧化死亡过程中抗原表达和定位的有力工具，可通过显微镜观察组织。在本章中，我们介绍常用的实验方案，并总结了在IHC和IF中常用的标记物用于监测铁过氧化死亡。©2023. 作者，独家授权给Springer Science+Business Media, LLC, 归Springer Nature旗下。

Ferroptosis is a type of regulated cell death driven by oxidative damage, characterized by iron overload and lipid peroxidation, and regulated by a network of distinct molecules and organelles. Impaired ferroptotic response is implicated in multiple physiological and pathological processes, including tumorigenesis, neurodegeneration, and ischemia-reperfusion damage. Classical techniques of immunohistochemistry (IHC) and immunofluorescence (IF) can be employed to exhibit antigen expression and location in tissues observed with microscopy, making them powerful tools in studying the ferroptosis process. In this chapter, we introduce commonly used protocols and summarize typical markers used in IHC and IF to monitor ferroptosis.© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.