微小RNA(miRNA)是一类参与生物过程的小型非编码RNA分子，包括发育、分化和疾病等。它们通过结合靶向mRNA的3'非翻译区(UTR)来实现mRNA降解或翻译抑制的功能。miRNA参与调控细胞增殖、凋亡和代谢等多个细胞过程。已经证明miRNA以多种方式调节铁依赖性细胞死亡（ferroptosis）。一些miRNA通过增加参与脂质过氧化的基因表达促进ferroptosis。其他miRNA通过减少参与铁吸收的基因表达抑制ferroptosis。尽管miRNA在ferroptosis中的作用仍在研究中，但它们在这一细胞死亡途径中扮演着重要角色。miRNA可能是与与ferroptosis相关的疾病（如癌症和神经退行性疾病）中的治疗干预的潜在靶点。本章概述了通过靶点发现和验证来研究miRNA与ferroptosis的关系的几种方法。© 2023. 该作者，Springer Science+Business Media, LLC - Springer Nature的独家许可下使用。

MicroRNAs (miRNAs) are a class of small non-coding RNA molecules that are involved in a wide range of biological processes, including development, differentiation, and disease. They function by binding to the 3' untranslated region (UTR) of target mRNAs, leading to mRNA degradation or translational repression. miRNAs are involved in the regulation of many cellular processes, including cell proliferation, apoptosis, and metabolism. MiRNAs have been shown to modulate ferroptosis in several ways. Some miRNAs have been shown to promote ferroptosis by increasing the expression of genes involved in lipid peroxidation. Other miRNAs have been shown to inhibit ferroptosis by decreasing the expression of genes involved in iron uptake. The role of miRNAs in ferroptosis is still being studied, but they play a significant role in this cell death pathway. miRNAs may be potential targets for therapeutic intervention in diseases associated with ferroptosis, such as cancer and neurodegenerative diseases. This chapter outlines several methods used to study the connection between miRNAs and ferroptosis through target discovery and validation.© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.