铁死是一种受铁依赖脂质过氧化驱动的非凋亡细胞死亡的调控形式。在过去的十年中，已报道铁死在中枢神经系统退行性疾病（例如，阿尔茨海默病、亨廷顿病和帕金森病）、中风、创伤性脑损伤和脑肿瘤中的病理作用。然而，如何可靠地检测和分类病理条件下的铁死与其他细胞死亡形式仍然是一个巨大的挑战，尤其是在原代脑细胞和脑组织中。本文总结了本研究中用于检测和分类脑中铁死的方法和协议（如实时PCR、Western blotting、免疫荧光染色、脂质过氧化测定试剂盒和探针、免疫荧光染色、GPX活性和谷胱甘肽耗尽测定试剂盒、铁检测和透射电镜技术）。 © 2023. 作者, 授予Springer Science+Business Media, LLC的独家许可, Springer Nature的一部分。

Ferroptosis is a regulated form of non-apoptotic cell death driven by iron-dependent lipid peroxidation. In the past decade, ferroptosis has been reported to be involved in the pathological role in the central nervous system degenerative diseases (e.g., Alzheimer's disease, Huntington's disease, and Parkinson's disease), stroke, traumatic brain injury, and brain tumor. However, how to reliably detect and classify ferroptosis from other cell death in pathological conditions remains a great challenge, especially in primary brain cells and brain tissues. Here, we summarize the methods and protocols (such as real-time PCR, western blotting, immunofluorescence staining, lipid peroxidation assay kits and probe, immunofluorescence staining, GPX activity and glutathione depletion assay kits, iron detection, and TEM) used in the present study to detect and classify ferroptosis in the brain.© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.