黑色素瘤抗原（MAGE）基因编码一系列具有共同MAGE同源结构域的蛋白质。这些基因在真核生物中保守，与包括泛素化和癌症中的致瘤过程在内的多种细胞和发育过程相关联。目前对MAGE蛋白质家族的认识主要来自对酵母和人类细胞系的分析，尚未有关于这些蛋白质在动物体内的有关功能报道。秀丽隐杆线虫（C. elegans）仅编码一个已知MAGE基因成员，mage-1（在酵母中称为NSE3），它是SMC-5/6复合物的组成部分。在这里，我们描述了mage-1/nse-3在秀丽隐杆线虫中有关有丝分裂和减数分裂的作用。mage-1/nse-3在减数分裂过程的亲姐妹重组修复中具有作用，并在遭受各种DNA损伤剂处理时，保持染色体完整性。MAGE-1直接与NSE-1和NSE-4相互作用。与smc-5、smc-6和nse-4突变体导致NSE-1核定位的丧失和强烈的细胞质积累相比，mage-1/nse-3突变体中NSE-1::GFP的水平降低，剩余的NSE-1::GFP部分核内但主要存在于细胞质中。我们的数据表明，MAGE-1对NSE-1的稳定性和SMC-5/6复合物的正常功能至关重要。©2023年作者。由牛津大学代表美国遗传学会出版。版权所有。有关权限，请发送电子邮件至：journals.permissions@oup.com。

Melanoma antigen (MAGE) genes encode for a family of proteins that share a common MAGE homology domain. These genes are conserved in eukaryotes, and have been linked to a variety of cellular and developmental processes including ubiquitination and oncogenesis in cancer. Current knowledge on the MAGE family of proteins mainly comes from the analysis of yeast and human cell lines, and their functions have not been reported at an organismal level in animals. C. elegans, only encodes one known MAGE gene member, mage-1 (NSE3 in yeast), forming part of the SMC-5/6 complex. Here, we characterize the role of mage-1/nse-3 in mitosis and meiosis in C. elegans. mage-1/nse-3 has a role in inter-sister recombination repair during meiotic recombination and for preserving chromosomal integrity upon treatment with a variety of DNA damaging agents. MAGE-1 directly interacts with NSE-1 and NSE-4. In contrast to smc-5, smc-6, and nse-4 mutants which cause the loss of NSE-1 nuclear localization and strong cytoplasmic accumulation, mage-1/nse-3 mutants have a reduced level of NSE-1::GFP, remnant NSE-1::GFP being partially nuclear but largely cytoplasmic. Our data suggest that MAGE-1 is essential for NSE-1 stability and the proper functioning of the SMC-5/6 complex.© The Author(s) 2023. Published by Oxford University Press on behalf of The Genetics Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.