糖类抗原15-3（CA15-3）是乳腺癌早期诊断的重要生物标志物。本文基于三维（3D）刺状核壳Au@PdCu纳米晶（标记的Au@PdCu NCs）构建了一种无标记电化学免疫传感器，用于高灵敏度检测CA15-3，其中K3[Fe(CN)6]作为电活性探针。Au@PdCu NCs通过简单的一锅湿化学方法合成，并通过多种技术对其形态、结构和电催化性能进行了研究。所制备的Au@PdCu NCs作为电极材料可以固定更多的抗体，作为信号放大材料通过其卓越的催化性能。所研发的生物传感器在最佳条件下显示出宽线性检测范围（0.1至300 U mL-1）和低检测限（0.011 U mL-1，S/N = 3）用于CA15-3的定量分析。所建立的生物传感平台为临床检测和早期诊断其他肿瘤生物标志物提供了一些见解。© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.

Carbohydrate antigen 15-3 (CA15-3) is an important biomarker for early diagnosis of breast cancer. Herein, a label-free electrochemical immunosensor was built based on three-dimensional (3D) urchin-like core-shell Au@PdCu nanocrystals (labeled Au@PdCu NCs) for highly sensitive detection of CA15-3, where K3[Fe(CN)6] behaved as an electroactive probe. The Au@PdCu NCs were synthesized by a simple one-pot wet-chemical approach and the morphology, structures, and electrocatalytic property were investigated by several techniques. The Au@PdCu NCs prepared worked as electrode material to anchor more antibodies and as signal magnification material by virtue of its exceptional catalytic property. The developed biosensor exhibited a wide linear detection range from 0.1 to 300 U mL-1 and a low limit of detection (0.011 U mL-1, S/N = 3) for determination of CA15-3 under the optimal conditions. The established biosensing platform exhibits some insights for detecting other tumor biomarkers in clinical assays and early diagnosis.© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.