已报道了夜间光照（LAN）引发的氧化应激与小鼠认知障碍有关。枸杞多糖（LBP）具有抗肿瘤、抗氧化和神经保护作用，然而其对光照引起的神经元损伤的神经保护效应尚不清楚。本研究发现，暴露于夜间光照的小鼠表现出认知障碍和抑郁样行为，而LBP治疗可以逆转这种现象。同时，LBP减轻了HT-22细胞中光照引起的较高凋亡和线粒体损伤。此外，LBP阻止了受光照处理的细胞中线粒体膜通透性（MMP）水平的降低。此外，LBP通过减少ROS产生和丙二醛（MDA）水平，同时增强超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）在受光照处理的小鼠和HT-22细胞中的水平，显示其抗氧化潜力。此外，受光照处理的小鼠和细胞中的Nrf2（NF-E2相关因子2）、血红素氧合酶1（HO-1）和NAD（P）H醌氧还原酶（NQO1）的mRNA和蛋白质表达均下降。此外，LBP治疗逆转了光照引起的Nrf2/HO-1信号通路抑制，无论是在小鼠还是细胞中。此外，Nrf2拮抗剂ML385在光照处理的细胞中显著消除了LBP对细胞凋亡、氧化应激和线粒体损伤的神经保护作用。这些结果表明，LBP通过激活Nrf2/HO-1信号通路，在小鼠和HT-22细胞中救治光照引起的神经毒性。版权所有 © 2023 Elsevier B.V. 发表。

Light at night (LAN) induced cognitive impairment associated with oxidative stress in mice has been reported. Lycium barbarum polysaccharide (LBP) exhibits anti-tumor, anti-oxidant and neuroprotective effects, yet the neuroprotective effect on light-induced neuron damage still unclear. Here, mice exposed to LAN displayed cognitive impairment and depressive like behavior, which was reversed by LBP treatment. Meanwhile, LBP alleviated light-induced higher apoptosis and mitochondrial damage in HT-22 cells. Also, LBP prevented the decreased of mitochondrial membrane permeabilization (MMP) level in light-treated cells. Additionally, LBP demonstrated its antioxidant potential by reducing ROS production and malondialdehyde (MDA) level, while simultaneously enhancing the levels of superoxide dismutase (SOD) and glutathione peroxidases (GSH-Px) in both light-treated mice and HT-22 cells. Furthermore, the mRNA and protein expression of Nrf2 (NF-E2-related factor 2), heme oxygenease-1 (HO-1), and NAD(P)H quinone oxidoreductase (NQO1) were decreased in both light-treated mice and cells. Additionally, LBP treatment reversed light-induced the inhibition of Nrf2/HO-1 signaling pathway in both mice and cells. Moreover, Nrf2 antagonist ML385 significantly eliminated the neuroprotection of LBP on cell apoptosis, oxidative stress and mitochondrial damage in light-treated cells. These results indicate that LBP can rescue light-induced neurotoxicity in mice and HT-22 cells by activating the Nrf2/HO-1 signaling pathway.Copyright © 2023. Published by Elsevier B.V.