评估天然生物质源是加速开发新型抗癌药物的一种有前途的策略。本研究旨在评估乌干德纳西斯（W. ugandensis）乙醇根和茎提取物对结直肠癌（CRC）细胞系（Caco-2）中五个靶基因（COX-2、CASPS-9、Bcl-xL、Bcl2和5-LOX）表达的活性。采用串行穷尽提取法得到植物提取物，并适当地用二甲基亚砜溶解以进行生物测定。Caco-2细胞系经过传代后，以不同浓度的植物提取物处理，并分离其RNA进行评估。我们独特的研究报告了乌干德纳西斯作为CRC生长的有效天然抑制剂，通过直接将其植物成分与COX-2、5-LOX、Bcl-xL、Bcl2的剂量依赖性下调和CASPS9基因的上调相关联。我们将W. ugandensis乙醇根和茎提取物作为有前景的CRC抑制剂，并建议进行体内和随后的临床试验，以验证其临床效果。此外，我们建议对乌干德纳西斯中参与调节机制，导致抑制CRC生长和可能的转移的具体代谢产物进行鉴定和表征的研究。版权所有© 2023作者。由Elsevier Masson SAS出版。保留所有权利。

The evaluation of natural biomass sources is a promising strategy in accelerating the development of novel anti-cancer medications. Our study aimed to evaluate the activity of W. ugandensis ethanolic roots and stems extracts on the expression of five targeted genes (COX-2, CASPS-9, Bcl-xL, Bcl2 and 5-LOX) in colorectal cancer (CRC) cell lines (Caco-2). Plant extracts were obtained using serial exhaustive extraction and dissolved in Dimethyl sulfoxide appropriately for bioassay. Caco-2 cell lines were passaged, treated with plant extracts at varying concentrations and their RNA's isolated for evaluation. Our unique study reports on W. ugandensis as efficient natural inhibitors of CRC growth, by directly linking its phytoconstituents to; downregulation of COX-2, 5-LOX, Bcl-xL, Bcl2 and upregulation of CASPS9 genes dose-dependently. We present W. ugandensis ethanolic roots and stems extracts as promising natural inhibitors for CRC carcinogenesis and recommend in vivo and subsequent clinical trials, with substantial clinical effects postulated. We further suggest studies on identification and characterization of the specific metabolites in W. ugandensis involved in the modulatory mechanisms, resulting to inhibition of CRC growth and possible metastases.Copyright © 2023 The Authors. Published by Elsevier Masson SAS.. All rights reserved.