肥胖与肠道渗透性增加及免疫反应减弱有关。 磷脂酰胆碱（PC）是鸡蛋中的一种胆碱形式，在肥胖的背景下，如果以磷脂酰胆碱为膳食中唯一的胆碱形式，已被显示有益地调节T细胞反应。 本研究旨在确定作为高脂饮食（HFD）一部分的不同剂量的磷脂酰胆碱对免疫细胞功能和肠道渗透性的影响。 4周龄的雄性Wistar大鼠被随机分为6组，在接下来的12周内消耗相同总胆碱量但胆碱形式不同的饮食：1- 控制低脂（CLF，20%脂肪，100％游离胆碱（FC））；2- 控制高脂（CHF，50%脂肪，100％FC）；3- 100% PC（100PC，50%脂肪，100％蛋-PC）；4- 75% PC（75PC，50%脂肪，75％蛋-PC + 25％FC）；5- 50% PC（50PC，50%脂肪，50％蛋-PC + 50％FC）；6- 25％PC（25PC；50%脂肪，25％蛋-PC + 75％FC） 通过FITC-dextran来测量肠道渗透性。 经不同促膜细胞刺激后，通过外体进行淋巴细胞和来自肠系膜淋巴结（MLN）的细胞的细胞因子产生来评估免疫功能。与CLF饮食相比，CHF饮食增加了肠道渗透性，并且50%或更高剂量的PC使渗透性恢复到与CLF饮食的水平相似。与CLF组相比，CHF饮食降低了脾细胞白细胞介素（IL）-1β、IL-2、IL-10和肿瘤坏死因子-alpha（TNF-α）的产生以及MLN白细胞介素-2（IL-2）的产生。50PC饮食最一致地显著改善了细胞因子水平（IL-2、IL-10、TNF-α）与CHF饮食相比。我们的结果表明，来源于蛋-PC的总胆碱剂量为50％可减轻HFD诱导的肠道渗透性和免疫细胞功能障碍。版权所有©2023 Elsevier Inc.发表。

Obesity is associated with increased intestinal permeability and a diminished immune response. Phosphatidylcholine (PC), a form of choline found in eggs, has been shown to beneficially modulate T cell response in the context of obesity when provided as the sole form of choline in the diet.This study aimed to determine the impact of varying doses of PC as part of a high-fat diet (HFD) on immune cell function and intestinal permeability.Male Wistar rats four weeks of age were randomized to consume one of six diets for 12 weeks containing the same amount of total choline but differing in the forms of choline: 1- Control Low-fat (CLF, 20% fat, 100% free choline (FC)); 2- Control High-fat (CHF, 50% fat, 100% FC); 3- 100% PC (100PC, 50% fat, 100% egg-PC); 4- 75% PC (75PC, 50% fat, 75% egg-PC+25% FC); 5- 50% PC (50PC, 50% fat, 50% egg-PC+50% FC); 6- 25% PC (25PC; 50% fat, 25% egg-PC+75% FC). Intestinal permeability was measured by FITC-dextran. Immune function was assessed by ex vivo cytokine production of splenocytes and cells isolated from the mesenteric lymph node (MLN) after stimulation with different mitogens.Feeding the CHF diet increased intestinal permeability compared to the CLF diet, and doses of PC 50% or greater returned permeability to levels similar to that of the CLF diet. Feeding the CHF diet lowered splenocyte production of interleukin (IL)-1β, IL-2, IL-10, and tumor necrosis factor-alpha (TNF-α), and MLN production of IL-2 compared to the CLF group. The 50PC diet most consistently significantly improved cytokine levels (IL-2, IL-10, TNF-α) compared to the CHF diet.Our results show that a dose of 50% of total choline derived from egg-PC can ameliorate HFD induced intestinal permeability and immune cell dysfunction.Copyright © 2023. Published by Elsevier Inc.