动脉粥样硬化是心血管疾病的主要原因。低密度脂蛋白（LDL）的积累，泡沫细胞的形成和动脉内膜的内皮功能障碍均对动脉粥样斑块的形成起到作用。认为大豆消耗对预防动脉粥样硬化有积极影响。因此，本研究对新型大豆多肽dglycin进行了纯化和表征。口服20 mg/g.d的dglycin可以使ApoE-/-小鼠主动脉根部动脉粥样斑块的病变面积减少了47.6 %，脂肪沉积减少了49.1 %。此外，它还降低了26.0 %的血浆低密度脂蛋白，27.2 %的甘油三酯，40.1 %的胆固醇，25.1 %的丙二醛和24.2 %的肿瘤坏死因子α（TNFα）水平。体外实验表明，dglycin通过抑制NF-κB信号传导途径，抑制了大动脉内皮细胞产生炎症细胞因子的能力。此外，它还抑制了活性氧自由基的产生，进一步增加了细胞存活率，并通过改善线粒体功能保护了大动脉内皮细胞免于坏死和凋亡。另一方面，dglycin通过抑制A1型清道夫受体的表达，阻止氧化LDL被巨噬细胞摄取，表明dglycin防止了泡沫细胞的形成。因此，dglycin通过抑制炎症、脂肪沉积，保护大动脉内皮细胞和防止泡沫细胞形成来减轻动脉粥样硬化的早期阶段.Copyright © 2023. Published by Elsevier B.V.

Atherosclerosis is a dominant cause of cardiovascular disease. Accumulation of low-density lipoproteins (LDL), formation of foam cells, and endothelial dysfunction within the arterial intima contribute to atherosclerotic plaque formation. Soy consumption is thought to have positive effect on the prevention of atherosclerosis. Therefore, in the present study, a novel soybean polypeptide dglycin was purified and characterized. Oral administration of 20 mg/g.d dglycin reduced 47.6 % lesion area, and 49.1 % lipid deposition in the atherosclerotic plaques in aortic roots in ApoE-/- mice. In addition, it decreased the levels of 26.0 % plasma low-density lipoprotein, 27.2 % triglyceride, 40.1 % cholesterol, 25.1 % malondialdehyde and 24.2 % tumor necrosis factor-alpha (TNFα). In vitro experiments revealed that dglycin inhibited inflammatory cytokine secretion from aortic endothelial cells via the inhibition of NF-κB signaling. Furthermore, it inhibited reactive oxygen species generation, subsequently enhanced cell viability, and protected aortic endothelial cells from necrosis and apoptosis via mitochondrial function improvement. On the other hand, dglycin prevented the uptake of oxidized LDL by macrophages via suppressing the expression of scavenger receptor class A1, which suggested that dglycin prevented foam cell formation. Therefore, dglycin alleviated the early-stage of atherosclerosis via depressing inflammation, lipid deposition, protecting aortic endothelial cells and preventing foam cell formation.Copyright © 2023. Published by Elsevier B.V.