从细胞免疫学角度确定雷公藤多糖苷（TGW）在银屑病性皮炎治疗中的作用。通过使用免疫活性剂imiquimod（IMQ）建立了银屑病性皮炎小鼠模型。根据随机数表将12只BALB/c小鼠分为IMQ组和IMQ+TGW组。通过半乳杜氏涂片染色评估体内的组织病理学变化。通过流式细胞术评估小鼠中的免疫细胞比例和细胞因子含量，以及体外PAM212细胞增殖情况。使用逆转录定量聚合酶链反应确定促炎细胞因子的表达。TGW显著改善了IMQ诱导的银屑病样小鼠皮肤病变的严重程度，并抑制了CD45+细胞、中性粒细胞和T淋巴细胞的激活（均P<0.01）。此外，TGW显著抑制了角质形成细胞（KCs）的增殖，并下调了炎症细胞因子，包括白细胞介素（IL）-17A、IL-23、肿瘤坏死因子α以及趋化因子（C-X-C moti）配体1的mRNA水平（P<0.01或P<0.05）。此外，它降低了小鼠皮损和引流淋巴结中γ δ T17细胞的数量（P<0.01）。TGW通过抑制KCs增殖以及相关免疫细胞和细胞因子的表达来改善类似银屑病的炎症。它抑制了γ δ T细胞的IL-17分泌，从而改善了银屑病的免疫炎症微环境。© 2023中国集成中西医杂志出版社和Springer-Verlag GmbH Germany，Springer Nature部分所有。

To determine the role of Tripterygium wilfordii multiglycoside (TGW) in the treatment of psoriatic dermatitis from a cellular immunological perspective.Mouse models of psoriatic dermatitis were established by imiquimod (IMQ). Twelve male BALB/c mice were assigned to IMQ or IMQ+TGW groups according to a random number table. Histopathological changes in vivo were assessed by hematoxylin and eosin staining. Ratios of immune cells and cytokines in mice, as well as PAM212 cell proliferation in vitro were assessed by flow cytometry. Pro-inflammatory cytokine expression was determined using reverse transcription quantitative polymerase chain reaction.TGW significantly ameliorated the severity of IMQ-induced psoriasis-like mouse skin lesions and restrained the activation of CD45+ cells, neutrophils and T lymphocytes (all P<0.01). Moreover, TGW significantly attenuated keratinocytes (KCs) proliferation and downregulated the mRNA levels of inflammatory cytokines including interleukin (IL)-17A, IL-23, tumor necrosis factor α, and chemokine (C-X-C motif) ligand 1 (P<0.01 or P<0.05). Furthermore, it reduced the number of γ δ T17 cells in skin lesion of mice and draining lymph nodes (P<0.01).TGW improved psoriasis-like inflammation by inhibiting KCs proliferation, as well as the associated immune cells and cytokine expression. It inhibited IL-17 secretion from γ δ T cells, which improved the immune-inflammatory microenvironment of psoriasis.© 2023. The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag GmbH Germany, part of Springer Nature.