已经证明，山金车（Arnica montana L.）能够缓解与创伤、手术后临床情况相关的炎症、疼痛和肿胀，但其作用机制尚不明确。本研究旨在调查山金车（A. montana）的母浸膏和高度稀释剂对不同细胞培养模型中炎症标记物、氧化应激和细胞迁移的影响。我们在人类和小鼠细胞培养模型中测试了山金车母浸膏和一系列高度稀释剂，通过测量炎症标记物：肿瘤坏死因子α（TNFα）、白细胞介素-6（IL-6）、环氧合酶-2（COX-2）、单核细胞趋化蛋白-1（MCP-1）、细胞间粘附分子（ICAM-1）、活性氧自由基（ROS）和细胞迁移，来证明它们的抗炎性能。采用酶联免疫吸附实验（ELISA法）测量炎症标记物。使用深红细胞ROX探针测量小胶质细胞内部的氧化应激（ROS）。使用Oris细胞迁移实验测量细胞迁移速度。这些数据表明，与车辆（对照组）相比，山金车（母浸膏和主要的1C稀释剂）能够显著降低发炎巨噬细胞中的TNFα产生。它们显著降低发炎的人类小胶质细胞中的IL-6和MCP-1，并显著降低发炎小鼠成纤维细胞中的COX-2表达。此外，山金车母浸膏减少了细胞迁移，而9C稀释剂与车辆相比，显著增强了成纤维细胞的迁移。山金车母浸膏和1C、3C、5C和9C稀释剂在发炎的人类内皮细胞中显著降低了ICAM-1的表达。山金车母浸膏和1C、3C、5C和9C稀释剂在发炎的小鼠小胶质细胞中引起了显著且一致的ROS产生效应。山金车1C对ROS产生的影响最大。山金车母浸膏和1C稀释剂在不同的人类和小鼠细胞模型中显示出抗炎性能，通过对数个标记物（促炎细胞因子、粘附分子、ROS）的测量证实。此外，山金车3C、5C和9C稀释剂在原代内皮细胞和小鼠小胶质细胞上表现出抗炎和抗氧化特性。版权所有 © 2023 Elsevier B.V. 发布。

The plant Arnica montana L. has been shown to alleviate inflammation, pain and swelling associated with trauma, and post-operative clinical conditions, yet the mechanism of action is not well understood.The study was designed to investigate the effect of Arnica montana (A. montana) mother tincture and homeopathic dilutions on inflammation markers, oxidative stress and cell migration in diverse cell culture models.We tested A. montana mother tincture and a range of homeopathic dilutions in different human and murine cell culture models to demonstrate their anti-inflammatory properties by measuring the inflammatory markers: tumor necrosis factor alpha (TNFα), interleukin-6 (IL-6), cyclooxygenase-2 (COX-2), monocyte chemoattractant protein-1 (MCP-1), intercellular adhesion molecule (ICAM-1) reactive oxygen species (ROS) and cell migration. The inflammatory markers were measured by ELISA assays. The intracellular oxidative stress (ROS) in microglial cells was measured using Deep Red CellROX probe. The cell migration was examined by wound healing using the Oris Cell migration assay.These data showed the ability of A. montana (mother tincture and mainly 1C dilution) to significantly reduce TNFα production in inflamed macrophages compared with vehicle (control). They significantly reduced both IL-6 and MCP-1 in inflamed human microglial cells and significantly decreased COX-2 expression in inflamed murine fibroblasts. Moreover, A. montana mother tincture reduced the cell migration whereas 9C dilution significantly enhanced the migration of fibroblast cells compared with vehicle. The expression of ICAM-1 was significantly reduced with A. montana mother tincture and 1C, 3C, 5C, and 9C dilutions in inflamed human endothelial cells compared with vehicle. A. montana mother tincture and 1C, 3C, 5C and 9C dilutions induced a significant and consistent effect on ROS production in inflamed murine microglial cells. A. montana 1C had the largest impact on ROS production.Mother tincture and 1C dilution of A. montana showed anti-inflammatory properties assessed by measurement of several markers (pro-inflammatory cytokines, adhesion molecule, ROS) in various human and murine cell models. In addition, A. montana 3C, 5C, 9C dilutions have anti-inflammatory and antioxidant effects as highlighted on both primary endothelial cells and murine microglial cells.Copyright © 2023. Published by Elsevier B.V.