结直肠癌是全球第三常见的癌症，也是第二致命的癌症。目前尚未研究过白蘑菇多糖的抗结直肠癌活性。本文中，我们使用常温水、热水、高压热水、稀碱性溶液和浓缩碱性溶液对白蘑菇多糖进行顺序提取。使用DEAE纤维素-52柱分离得到纯化的多糖（WAAP-1）。对WAAP-1进行了物理化学性质、结构特征和抗结直肠癌活性的研究。结果显示，WAAP-1是一种中性多糖，分子量为10.1 kDa。其中的单糖组成为葡萄糖、甘露糖和半乳糖，其摩尔比为84.95:8.97:4.50。主要链由(1,4)-α-D-Glcp和(1,6)-β-D-Manp组成。体外抗结直肠癌实验表明，WAAP-1可以显著抑制结肠癌细胞HT-29的增殖。它通过上调Caspase-3、Bax和E-cadherin蛋白的表达以及下调Bcl-2和Vimentin蛋白的表达促进细胞凋亡和抑制上皮间质转化。这些结果为开发新型功能食品或抗肿瘤药物提供了新的潜在可能性。版权所有©2023 Elsevier B.V.发表。

Colon cancer is the third most prevalent cancer and the second most deadly cancer in the world. Anti-colon cancer activity of Agaricus bisporus polysaccharides has not been studied. In this paper, Agaricus bisporus polysaccharides were sequentially extracted by room temperature water, hot water, high pressure hot water, dilute alkaline solution and concentrated alkaline solution. A homogeneous polysaccharide (WAAP-1) was obtained using DEAE Cellulose-52 column. Physicochemical properties, structural characterization and anti-colon cancer activity of WAAP-1 were investigated. The results showed that WAAP-1 was a neutral polysaccharide with molecular weight of 10.1 kDa. The monosaccharide composition was glucose, mannose and galactose with a molar ratio of 84.95:8.97:4.50. The main chain was mainly composed of (1,4)-α-D-Glcp and (1,6)-β-D-Manp. In vitro anti-colon cancer results showed that WAAP-1 could significantly inhibit proliferation of colon cancer cell HT-29. It promoted apoptosis and inhibited epithelial mesenchymal transition of HT-29 by up-regulating the expression of Caspase-3, Bax and E-cadherin proteins and down-regulating the expression of Bcl-2 and Vimentin proteins. The results provided new potential possibilities for the development of novel functional foods or antitumor drugs.Copyright © 2023. Published by Elsevier B.V.