口腔癌是欧洲最常见的癌症之一，其广泛传播需要开发低成本和可靠的便携式平台用于诊断，并具备高选择性和敏感性的特征。本研究报道了基于分子印迹聚合物（MIP）的电化学传感器在检测TGF-β1中的应用的开发和表征。优化后的生物传感器是口腔癌早期筛查的一个潜在工具。通过在铂电极上以TGF-β1作为模板分子，在间苯二胺（o-PD）存在下进行电聚合生成了一种仿生表面。分子印迹聚合物的合成、模板去除和TGF-β1再结合过程通过差示脉冲伏安法（DPV）进行监测。原子力显微镜（AFM）用于研究和表征表面形貌，并研究洗涤步骤对MIP和NIP（非印迹聚合物，作为对照）的影响，聚合物层的厚度通过扫描透射电子显微镜（STEM）分析进行测量。MIP传感器的性能在缓冲溶液和唾液样品中进行了测试，显示出在考虑范围（从20 ng ml-1到0.5 ng ml-1）内的线性响应，LOD为0.09 ng mL-1，对TGF-β1具有亲和力和选择性，即使在存在干扰分子的情况下也是如此。该传感器还用于检测含有靶分子的唾液样品，显示出良好的恢复结果，表明该系统可能用于口腔癌诊断的大规模快速筛查。

Oral cancer is one of the most common types of cancer in Europe and its large diffusion requires, together with prevention, the development of low-cost and reliable portable platforms for its diagnosis, with features of high selectivity and sensitivity. In this study, the development and characterization of a molecularly imprinted polymer (MIP)-based electrochemical sensor for TGF-β1 detection are reported. The optimized biosensor is a potential tool for the early screening of oral cancer. A biomimetic surface has been obtained by electropolymerization of o-phenylenediamine (o-PD) on platinum electrodes, in the presence of TGF-β1 as a template molecule. MIP synthesis, template removal and TGF-β1 rebinding have been monitored by Differential Pulse Voltammetry (DPV). Atomic Force Microscopy (AFM) has been performed to investigate and characterize the surface morphology and the influence of the washing step on MIP and NIP (non-imprinted polymer as the control) while the thickness of the polymer layer has been measured by Scanning Transmission Electron Microscopy (STEM) analysis. The MIP sensor performance has been tested in both buffer solution and saliva samples with TGF-β1, showing a linear response in the considered range (from 20 ng ml-1 down to 0.5 ng ml-1), an outstanding LOD of 0.09 ng mL-1 and affinity and selectivity to TGF-β1 also in the presence of interfering molecules. The sensor was used also for the detection of target molecules in spiked saliva samples with good recovery results suggesting the possibility of the use of the proposed system for large scale fast screening in oral cancer diagnosis.