胰腺癌是一种具有高死亡率和缺乏有效治疗方法的毁灭性疾病。早期检测的挑战以及胰腺癌的高度侵袭性影响了治疗选择，突显出改进疾病修饰疗法的紧迫需求。基于肽的生物医药制剂由于其优势特性，如高选择性和亲和性、化学可修饰性、良好的组织渗透性以及易于代谢和排泄而成为一个备受关注的研究领域。噬菌体展示是一个能够鉴定具有高亲和力和特异性的肽对靶分子的强大技术，已经成为发现基于肽的药物的关键工具。噬菌体展示技术涉及使用噬菌体表示肽库，然后对感兴趣的靶点进行筛选以识别具有所需特性的肽。这种方法在癌症诊断和治疗中显示了巨大的潜力，并可应用于肿瘤细胞靶向和新疗法的开发。在本综述中，我们提供了噬菌体载体的基本生物学概述，噬菌体库构建原理以及不同的结合亲和力评估方法。然后，我们描述了噬菌体展示在胰腺癌治疗、靶向药物输送和早期检测中的应用。尽管有着巨大的潜力，但仍然存在一些待解决的挑战，如优化选择过程和改进基于噬菌体的药物的药代动力学特性等。然而，噬菌体展示为胰腺癌和其他肿瘤的新型靶向治疗方法的开发提供了一种有希望的途径。版权所有 © 2023年李，杨，段，杜和叶。

Pancreatic cancer is a devastating disease with a high mortality rate and a lack of effective therapies. The challenges associated with early detection and the highly aggressive nature of pancreatic cancer have limited treatment options, underscoring the urgent need for better disease-modifying therapies. Peptide-based biotherapeutics have become an attractive area of research due to their favorable properties such as high selectivity and affinity, chemical modifiability, good tissue permeability, and easy metabolism and excretion. Phage display, a powerful technique for identifying peptides with high affinity and specificity for their target molecules, has emerged as a key tool in the discovery of peptide-based drugs. Phage display technology involves the use of bacteriophages to express peptide libraries, which are then screened against a target of interest to identify peptides with desired properties. This approach has shown great promise in cancer diagnosis and treatment, with potential applications in targeting cancer cells and developing new therapies. In this comprehensive review, we provide an overview of the basic biology of phage vectors, the principles of phage library construction, and various methods for binding affinity assessment. We then describe the applications of phage display in pancreatic cancer therapy, targeted drug delivery, and early detection. Despite its promising potential, there are still challenges to be addressed, such as optimizing the selection process and improving the pharmacokinetic properties of phage-based drugs. Nevertheless, phage display represents a promising approach for the development of novel targeted therapies in pancreatic cancer and other tumors.Copyright © 2023 Li, Yang, Duan, Du and Ye.