通过免疫库NGS评估急性淋巴细胞白血病的最低残余疾病需要在患者样本中添加已知数量的CDR3序列。最近，EuroClonality-NGS组发布了最全面的该目的的方案之一。ARResT/Interrogate是这一团队开发的用于处理这些NGS文库的闭源软件。Vidjil是一种开源的替代方法，目前无法处理使用这种方案制备的文库。在这里，我们提供了一个Vidjil附加模块来解决这个问题。通过Vidjil和ARResT/Interrogate分析EuroClonality-NGS制备的样本，结果高度一致（r = 0.998），并且误差较低（均方根误差，RMSE = 0.112）。© 2023年作者。由英国血液学会和约翰·威立出版社出版的eJHaem。

Assessment of minimal residual disease in acute lymphoblastic leukemia by immune repertoire NGS requires spiking CDR3 sequences at known quantities into the patient's sample. Recently, the EuroClonality-NGS group released one of the most comprehensive protocols for this purpose. ARResT/Interrogate is a closed-source software for processing these NGS libraries, developed by this same group. Vidjil, an open-source alternative, currently cannot handle libraries prepared using this protocol. Here, we present a Vidjil add-on to solve this issue. EuroClonality-NGS prepared samples analyzed with Vidjil and ARResT/Interrogate were highly concordant (r = 0.998) and presented low error (root-mean-square error, RMSE = 0.112).© 2023 The Authors. eJHaem published by British Society for Haematology and John Wiley & Sons Ltd.