N6-甲基腺嘌呤（m6A）在调控基因表达中起到重要作用，并与多种疾病有关。然而，它在内皮祖细胞（EPCs）分化中的作用尚不清楚。本研究使用液相色谱串联质谱和免疫荧光分析法来定量人外周血源性EPCs（HPB-EPCs）在分化为成熟细胞前后的m6A水平。本研究利用细胞计数试剂盒8、Transwell和管形成实验来确定Wilms瘤1相关蛋白（WTAP）的过表达和抑制对HPB-EPCs的影响。结果显示，m6A修饰水平在HPB-EPCs分化过程中显著增加，其中WTAP在m6A调控中表现出最显著的变化。当WTAP在HPB-EPCs中过表达时，细胞增殖、侵袭和血管生成均得到改善，而WTAP抑制产生相反效果。综上所述，本研究凸显了m6A在调控EPCs分化中的作用，其中WTAP作为EPCs分化的促进剂。版权：©伍等。

N6-methyladenosine (m6A) serves a critical role in regulating gene expression and has been associated with various diseases; however, its role in the differentiation of endothelial progenitor cells (EPCs) remains unclear. The present study used liquid chromatography with tandem mass spectrometry and immunofluorescence assays to quantify the levels of m6A in human peripheral blood-derived EPCs (HPB-EPCs) before and after differentiation into mature cells. The present study performed Cell Counting Kit 8, Transwell, and tube formation assays to determine the effects of overexpression and knockdown of Wilms' tumor 1-associated protein (WTAP) on HPB-EPCs. The results revealed that the level of m6A modification was significantly increased during HPB-EPCs differentiation, and WTAP exhibited the most significant alteration among the enzymes involved in m6A regulation. When WTAP was overexpressed in HPB-EPCs, cell proliferation, invasion, and the formation of tubes were improved, whereas WTAP knockdown yielded the opposite effects. In conclusion, the present study highlighted the involvement of m6A in regulating EPC differentiation, with WTAP acting as a promoter of EPC differentiation.Copyright: © Wu et al.