皮质释放激素受体-1（CRH-R1）在人体嗜酸性粒细胞中有表达，但其在皮肤疾病中的作用尚不明确。通过使用顺序双染技术，研究了CRH-R1在太阳角化症（AK）、基底细胞癌（BCC）、鳞状细胞癌（SCC）和银屑病患者的病损和非病损皮肤样本中嗜酸性粒细胞的表达。在所有患者组的非病损皮肤中，真皮组胺酶阳性嗜酸性粒细胞表达CRH-R1免疫反应性。在AK（p = 0.03）和银屑病（p = 0.02）的病损皮肤中，CRH-R1的表达显著增加，BCC（p = 0.129）中表达不增加，而在SCC中未增加。为了研究CRH-R1的调节，在LAD2嗜酸性粒细胞系中用紫外线B（UVB）照射或CRH或1,25-二羟基维生素D3 [1,25-(OH)2 D3]刺激。结果表明，90 mJ/cm2的UVB（p = 0.041）和120 mJ/cm2的UVB（p = 0.039）会降低CRH-R1的表达。相反，100和1000 nM的CRH会增加CRH-R1的免疫染色，但对增殖反应没有影响。10和100 nM的1,25-(OH)2 D3处理会明显增加CRH-R1染色。在经过UVB照射后，CRH浓度会增加到条件培养基中，但在超声破碎的LAD2嗜酸性粒细胞中没有增加。总之，嗜酸性粒细胞中CRH-R1水平不足可能与SCC和可能与BCC的抗肿瘤反应减弱有关。© 2023 The Authors. Experimental Dermatology published by John Wiley & Sons Ltd.

Corticotropin-releasing hormone receptor-1 (CRH-R1) is expressed in human mast cells, but its role in skin diseases is unknown. By using a sequential double-staining technique, the mast cell expression of CRH-R1 was investigated in biopsies from lesional and non-lesional skin samples of patients with actinic keratosis (AK), basal cell carcinoma (BCC), squamous cell carcinoma (SCC) and psoriasis. Dermal tryptase+ mast cells expressed CRH-R1 immunoreactivity in the non-lesional skin in all patient groups. The CRH-R1 expression was significantly increased in the lesional skin of AK (p = 0.03) and psoriasis (p = 0.02), non-significantly in BCC (p = 0.129), but not increased in SCC. To investigate the regulation of CRH-R1, the LAD2 mast cell line was irradiated with UVB or stimulated with CRH or 1,25-dihydroxyvitamin D3 [1,25-(OH)2 D3 ]. Consequently, UVB at 90 mJ/cm2 (p = 0.041) and 120 mJ/cm2 (p = 0.039) decreased CRH-R1 expression. Instead, CRH at 100 and 1000 nM increased CRH-R1 immunostaining, but did not affect the proliferative response. The treatment with 10 and 100 nM 1,25-(OH)2 D3 led to a noticeable increase in CRH-R1 staining. After irradiating with UVB, the concentration of CRH increased in the conditioned medium, but not in sonicated LAD2 mast cells. In conclusion, the lack of sufficient levels of CRH-R1 in mast cells may be related to diminished antitumoural response in SCC and possibly in BCC.© 2023 The Authors. Experimental Dermatology published by John Wiley & Sons Ltd.