缺血性中风是由于大脑供血不足引起的。其具有急性发作、常伴有意识障碍以及较高的死亡率和残疾率。然而，核糖体蛋白（RP）-S15与线粒体核糖体蛋白（MRP）-S27与缺血性中风之间的关系尚不清楚。从GPL6883、GPL11154和GPL570产生的基因表达数据库GSE22255、GSE16561和GSE199435中下载了数据。筛选了差异表达基因（DEGs），并进行了蛋白质相互作用网络的构建和分析、功能富集分析以及基因集富集分析。绘制了基因表达热图。执行了比较毒理基因组数据库分析，以找到与核心基因相关的疾病。TargetScan筛选了调控中心DEGs的miRNAs。鉴定了500个DEGs。根据基因本体分析，发现它们主要富集于白细胞活化、免疫反应中的肌细胞活化、细胞膜、线粒体、分泌囊泡、催化活性、酶结合、核糖核酸结合和剪接。基因集富集分析显示富集项与差异表达基因的富集项相似。获得了20个核心基因。比较毒理基因组数据库分析显示，6个基因（RPS15、RPS2、RPS3、MRPS27、POLR2A、MRPS26）与化学药物引起的肝损伤、坏死、延迟围产期暴露、肾病、肝脏肿大和肿瘤有关。RPS15和MRPS27是缺血性中风的核心基因，在缺血性中风中起着重要作用。版权所有©2023年作者。由Wolters Kluwer Health, Inc.发表。

Ischemic stroke is caused by insufficient blood supply to the brain. It has acute onset, often disturbance of consciousness, and high mortality and disability rate. However, relationship between ribosomal proteins (RP)-S15 and mitochondrial ribosomal proteins (MRP)-S27 and ischemic stroke remains unclear. The ischemic stroke datasets GSE22255, GSE16561, and GSE199435 were downloaded from gene expression omnibus generated by GPL6883, GPL11154, and GPL570. Differentially expressed genes (DEGs) were screened, and the construction and analysis of protein-protein interaction network, functional enrichment analysis and gene set enrichment analysis were performed. The gene expression heat map was drawn. Comparative toxicogenomics database analysis were performed to find the disease most related to core gene. TargetScan screened miRNAs that regulated central DEGs. Five hundred DEGs were identified. According to gene ontology analysis, they were mainly enriched in leukocyte activation, myoid cell activation involved in immune response, cell membrane, mitochondria, secretory vesicles, catalytic activity, enzyme binding, ribonucleic acid binding, splicing. Gene set enrichment analysis showed that the enrichment items are similar to the enrichment items of differentially expressed genes. And 20 core genes were obtained. Comparative toxicogenomics database analysis showed that 6 genes (RPS15, RPS2, RPS3, MRPS27, POLR2A, MRPS26) were found to be associated with chemical and drug-induced liver injury, necrosis, delayed prenatal exposure, nephropathy, hepatomegaly and tumor. RPS15 and MRPS27 are the core genes of ischemic stroke and play an important role in ischemic stroke.Copyright © 2023 the Author(s). Published by Wolters Kluwer Health, Inc.