产前操作可能导致后代神经行为的改变。本研究考察了妊娠期间强制运动和锌补充对产前束缚应激（PRS）诱发的行为障碍、神经炎症反应和氧化应激方面的个体和综合效应。将孕鼠分为五组：对照组；束缚应激组（RS）；RS + 运动应激组（RS + ES）；RS + 锌补充组（RS + Zn）；RS + ES + Zn组。除对照组外，所有孕鼠在妊娠第15天至第19天接受了束缚应激。ES组孕鼠接受了强制跑步机运动（每天30分钟），Zn组孕鼠接受了硫酸锌补充（每天口服30毫克/千克），并持续至妊娠期结束。在25-27日龄，记录了焦虑样和应对压力的行为，并测量了前额叶皮层中白介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）的基因表达，以及总抗氧化能力的浓度。PRS显著增加了焦虑，产生了被动应对行为，增加了IL-1β和TNF-α的表达，并降低了抗氧化能力。ES增强了PRS诱发的行为障碍，而锌则逆转了该效应。产前锌还恢复了抗炎和抗氧化能力，但对RS和ES的合并效应无影响。锌抑制了PRS诱导的行为和神经生物学损害，显示了锌对PRS引起的儿童气质变化的临床重要性的潜在意义。© 2023 Wiley Periodicals LLC.

Prenatal manipulations can lead to neurobehavioral changes in the offspring. In this study, individual and combined effects of forced exercise and zinc supplementation during pregnancy on prenatally restraint stress (PRS)-induced behavioral impairments, neuro-inflammatory responses, and oxidative stress have been investigated in adolescent female rat offspring. Pregnant rats were divided into five groups: control; restraint stress (RS); RS + exercise stress (RS + ES), RS + zinc supplementation (RS + Zn); and RS + ES + Zn. All the pregnant rats (except control) were exposed to RS from gestational days 15 to 19. Pregnant rats in ES groups were subjected to forced treadmill exercise (30 min/daily), and in Zn groups to zinc sulfate (30 mg/kg/orally), throughout the pregnancy. At postnatal days 25-27, anxiety-like and stress-coping behaviors were recorded, and the gene expressions of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) and the concentration of total antioxidant capacity were measured in the prefrontal cortex. PRS significantly enhanced anxiety, generated passive coping behaviors, increased IL-1β and TNF-α expression, and decreased the antioxidant capacity. ES potentiated while zinc reversed PRS-induced behavioral impairments. Prenatal zinc also restored the anti-inflammatory and antioxidant capacity but had no effect on additive responses imposed by the combination of RS and ES. Suppression of PRS-induced behavioral and neurobiological impairments by zinc suggests the probable clinical importance of zinc on PRS-induced changes on child temperament.© 2023 Wiley Periodicals LLC.