本研究首次采用经济实惠和环保的方法，利用棉籽饼和牛乳酵母提取物对银纳米颗粒（AgNPs）和金纳米颗粒（AuNPs）进行了绿色合成。通过UV-Vis光谱、动态光散射分析（DLS）、透射电子显微镜（TEM）、选择区域电子衍射（SAED）和傅里叶变换红外光谱（FTIR）对生物合成的纳米颗粒进行了表征。此外，还对生物合成的纳米颗粒进行了对负性细菌（大肠杆菌、奇异变形杆菌、克雷伯氏菌、沙门氏菌和铜绿假单胞菌）、金黄色葡萄球菌以及抗人类腺病毒第5型（HAdV-5）和HepG2肝细胞癌细胞的体外生物膜形成和抗癌活性测试。银纳米颗粒和金纳米颗粒的UV-Vis吸收光谱在440nm和540nm处出现了最大吸光度。DLS测量结果表明，银纳米颗粒和金纳米颗粒的最高强度分别为84nm和73.9nm。FTIR测量确定了棉籽饼和牛乳酵母提取物中有一些功能性基团能够将银离子和金离子还原为金属银和金。通过TEM和SAED图案分析，确认了银纳米颗粒和金纳米颗粒的形态和粒径。生物合成的银纳米颗粒和金纳米颗粒对大肠杆菌、奇异变形杆菌、克雷伯氏菌、沙门氏菌、铜绿假单胞菌和金黄色葡萄球菌所产生的生物膜具有良好的抑制作用。此外，它们对人类肝癌细胞（HepG-2）的抗癌活性和抗人腺病毒第5型感染的抗病毒活性也有一定作用。最后，本研究结果对纳米生物技术、制药和食品包装领域具有非常显著的价值，可以通过开发环保和经济的多功能纳米颗粒来开发抗菌和/或抗癌药物。

For the first time in this study, silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) were green synthesized by the cost-effective and eco-friendly procedure using Cotton seed meal and Fodder yeast extracts. The biosynthesized NPs were characterized by UV-Vis spectroscopy, dynamic light scattering analysis (DLS), transmission electron microscopy (TEM), selected area electron diffraction (SAED), and fourier-transform infrared (FTIR) spectroscopy. Furthermore, the biosynthesized NPs were tested in vitro against biofilm formation by some pathogenic negative bacteria (Escherichia coli, Proteus mirabilis, Klebsiella sp., Salmonella sp., and Pseudomonas aeruginosa) and negative bacteria (staphylococcus aureus) as well as against human denovirus serotype 5 (HAdV-5) and anticancer activity using HepG2 hepatocarcinoma cells. UV-Vis absorption spectra of reaction mixture of AgNPs and AuNPs exhibited maximum absorbance at 440 nm and 540 nm, respectively. This finding was confirmed by DLS measurements that the highest intensity of the AgNPs and AuNPs were 84 nm and 73.9 nm, respectively. FTIR measurements identified some functional groups detected in Cotton seed meal and Fodder yeast extracts that could be responsible for reduction of silver and gold ions to metallic silver and gold. The morphologies and particle size of AgNPs and AuNPs were confirmed by the TEM and SAED pattern analysis. Biosynthesized AgNPs and AuNPs showed good inhibitory effects against biofilms produced by Escherichia coli, Proteus mirabilis, Klebsiella sp., Salmonella sp., Pseudomonas aeruginosa, and Staphylococcus aureus. In addition, they showed anticancer activities against hepatocellular carcinoma (HepG-2) and antiviral activity against human adenovirus serotype 5 infection in vitro. Finally, the results of this study is expected to be extremely helpful to nano-biotechnology, pharmaceutical, and food packing applications through developing antimicrobial and/or an anticancer drugs from ecofriendly and inexpensive nanoparticles with multi-potentiality.