化学发光探针已经在精确肿瘤成像和免疫荧光分析等多个研究领域中越来越受欢迎。然而，以往开发的化学发光探针主要局限于研究与氧化反应相关的生物事件。本研究首次提出了应用于生物成像的可调发射波长范围从525到800纳米的双环二氧化锂化学发光探针的例子。这些新开发的探针能够具有高特异性和检测限度为77毫单元/升、96纳摩尔和28纳摩尔的能力检测β-半乳糖苷酶、H2O2和超氧化物。探针的生物成像应用已在卵巢癌细胞、肝癌细胞和巨噬细胞上得到验证，能够检测细胞内β-半乳糖苷酶、H2O2和超氧化物的含量。高特异性使我们能够成像移植瘤在小鼠身上的位置。我们预计我们的探针将在使用非侵入性成像技术记录复杂的生物分子事件方面得到广泛应用。

Chemiluminescent probes have become increasingly popular in various research areas including precise tumor imaging and immunofluorescence analysis. Nevertheless, previously developed chemiluminescence probes are mainly limited to studying oxidation reaction-associated biological events. This study presents the first example of bioimaging applicable bicyclic dioxetane chemiluminescent probes with tunable emission wavelengths that range from 525 to 800 nm. These newly developed probes were able to detect the analytes of β-Gal, H2O2, and superoxide with high specificity and a limit of detection of 77 mU L-1, 96, and 28 nM, respectively. The bioimaging application of the probes was verified in ovarian and liver cancer cells and macrophage cells, allowing the detection of the content of β-Gal, H2O2, and superoxide inside the cells. The high specificity allowed us to image the xenografted tumor in mice. We expect that our probes will receive extensive applications in recording complex biomolecular events using noninvasive imaging techniques.