药用植物是无数微生物的寄主，通常被称为内生菌，其含有丰富的生物活性物质，具有有益生物活性。内生菌以促进宿主植物中次生代谢物的积累而对其产生深远影响，这种代谢物对人类有益。本研究通过在固体稻米培养基上进行发酵，评估了从药用植物藤黄属的真菌内生菌ABR4中提取的生物活性次生代谢物。使用溶剂提取法对ABR4真菌提取物进行了顺序纯化，并使用TLC、UV光谱分析、HRESI-MS、FTIR和GC-MS分析等不同的光谱和分析技术进行了表征。GC-MS分析揭示了有吡啶、苯甲酸、4-[(三甲基硅基)氧基]-三甲基硅酯、十六烷酸三甲基硅酯和油酸三甲基硅酯等物质的存在。通过MTT测定评估了ABR4的细胞毒性对肺癌（A549）和乳腺癌（MCF-7）细胞系。这些化合物对测试的细胞系没有显著的细胞毒性。通过记录47%到54%的抑制率，评估了ABR4的内生菌提取物在对人类病原菌（金黄色葡萄球菌、脑膜炎杆菌、大肠杆菌、伤寒沙门氏菌、铜绿假单胞菌和白色念珠菌）的抗菌潜力。总之，内生菌菌株ABR4表现出显著的抗菌活性对测试的病原菌有效。此外，从内生菌菌株ABR4中分离的功能性代谢物表明其作为抗菌剂在制药工业中具有更广泛的用途。© 2023. 版权所有，由Springer Science+Business Media, LLC独家授权，Springer Nature的一部分。

Medicinal plants are hosts to an infinite number of microorganisms, commonly referred to as endophytes which are rich in bioactive metabolites yielding favorable biological activities. The endophytes are known to have a profound impact on their host plant by promoting the accumulation of secondary metabolites which are beneficial to humankind. In the present study, the fungal endophyte, Fusarium solani (ABR4) from the medicinal plant Tinospora cordifolia, was assessed for its bioactive secondary metabolites employing fermentation on a solid rice medium. The crude ABR4 fungal extract was sequentially purified using the solvent extraction method and characterized using different spectroscopic and analytical techniques namely TLC, UV spectroscopic analysis, HRESI-MS, FTIR, and GC-MS analysis. The GC-MS analysis revealed the presence of pyridine, benzoic acid, 4-[(trimethylsilyl)oxy]-trimethylsilyl ester, hexadecanoic acid trimethylsilyl ester, and oleic acid trimethylsilyl ester. The cytotoxic ability of ABR4 was evaluated by MTT assay against lung cancer (A549) and breast cancer (MCF-7) cell lines. The compounds did not exhibit significant cytotoxicity against the tested cell lines. The endophytic ABR4 extract was evaluated for its antimicrobial potential against human pathogens (S. aureus, B. cereus, E. coli, S. typhimurium, P. aeruginosa, and C. albicans) by recording 47 to 54% inhibition. Taken together, the endophytic fungal strain ABR4 demonstrated a remarkable antimicrobial activity against the tested pathogens. Furthermore, the functional metabolites isolated from the endophytic strain ABR4 reveal its broader usage as antimicrobial agents for newer drug development in the pharmaceutical industry.© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.