最近的研究中报告称，使用整草提取物可以协同增强治疗药物的抗癌活性。本研究评估了印度草（Smilax corbularia Kunth，以下简称S. corbularia）和凤尾菇（Phellinus linteus，以下简称P. linteus）的粗提取物的抗氧化和抗癌活性，包括单独使用和混合使用。S. corbularia的提取采用乙醇，而P. linteus的提取采用热水。两种粗提取物均进行了理化特性表征。随后，使用2,2-二苯基-1-苦肼和2,2'-联苯并(3-乙基苯并噻唑啉-6-磺酸)试剂对这两种粗提取物的抗氧化活性进行了评估。然后，对它们对乳腺癌细胞的细胞毒性、增殖和凋亡的影响进行了评估。结果显示，得到的S. corbularia粗提取物总酚含量较高，而P. linteus的粗提取物则具有较高的总多糖含量。混合组合的总酚含量和总多糖含量结果取决于各个提取物的比例。单独使用S. corbularia和组合3（含75% S. corbularia：25% P. linteus）表现出最强的自由基清除活性，其次是组合1（50% S. corbularia：50% P. linteus）、组合2（25% S. corbularia：75% P. linteus）和P. linteus。提取物样品对癌细胞的毒性结果与其抗氧化活性相一致。特别是，某些组合对三种类型的乳腺癌细胞的增殖具有更明显的抑制作用，而这些作用与两种单独提取物相比更为显著。然而，诱导凋亡的效果有限，凋亡程度观察到为＜5%。这些发现表明，使用这两种提取物的混合物对乳腺癌细胞可能具有增强的抗氧化和抑制增殖效果。因此，这两种提取物的联合作为抗癌药物的潜力值得进一步研究。版权所有：© Syukriya等人。

Treatment with extracts from whole herbs has been reported to synergistically enhance the anticancer activities of therapeutic agents in recent studies. The present study evaluated the antioxidant and anticancer activities of Smilax corbularia Kunth (S. corbularia) and Phellinus linteus (P. linteus) crude extracts individually and in combination. S. corbularia was extracted using ethanol, whereas P. linteus was extracted using hot water. Both crude extracts underwent physiochemical characterization. Subsequently, the possible antioxidant activities of both crude extracts, individually and in combination, were evaluated using 2,2-Diphenyl-1-picrylhydrazyl and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) assays. Their effects on breast cancer cell cytotoxicity, proliferation and apoptosis were then assessed. The crude S. corbularia extract obtained was found to have a high level of total phenolic content, whilst the crude P. linteus extract had high levels of total polysaccharide content. The total phenolic content and total polysaccharide content results of the combinations depended on the respective ratios of the individual extracts. S. corbularia alone and combination 3 (which contained 75% S. corbularia: 25% P. linteus) demonstrated the greatest radical scavenging activity, followed by combination 1 (50% S. corbularia: 50% P. linteus), combination 2 (25% S. corbularia: 75% P. linteus) and P. linteus. The toxicity results of the extract samples on the cancer cells corresponded with their antioxidant activity. In particular, certain combinations demonstrated clearer inhibitory effects on cell proliferation against three types of breast cancer cells compared with those exerted by the two individual extracts. However, induction of apoptosis was limited, with the degree of apoptosis observed to be #x003C;5%. These findings suggested that treatment with combinations of these two extracts could confer enhanced antioxidant and antiproliferative effects on breast cancer cells. Therefore, the potential of these two extracts in combination as anticancer agents warrants further investigation.Copyright: © Syukriya et al.