为了确定乳酸菌菌株及其组合在抑制胃幽门螺杆菌（H. pylori）的定殖和胃黏膜炎症发生中的作用，将人类胃腺癌AGS细胞与H. pylori和六种益生菌菌株（乳酸菌酸杆菌NCFM、乳酸菌酸杆菌La-14、乳酸植物杆菌Lp-115、乳酸酪榭杆菌Lpc-37、乳酸酪榭杆菌Lr-32和乳酸酪榭杆菌GG）共同培养，并使用荧光显微镜和尿素酶活性测定法评估不同组合中H. pylori的粘附能力。将雄性C57BL/6小鼠随机分为五组（未感染组、H. pylori组、H. pylori+NCFM组、H. pylori+Lp-115组和H. pylori+NCFM+Lp-115组），并连续六周给予两种乳酸菌菌株（NCFM和Lp-115）处理。使用快速尿素酶试验、苏木精-伊红（HE）染色、免疫组化、qRT-PCR和ELISA确定H. pylori定殖和组织炎症状态。 乳酸菌酸杆菌NCFM、乳酸菌酸杆菌La-14、乳酸植物杆菌Lp-115、乳酸酪榭杆菌Lpc-37、乳酸酪榭杆菌Lr-32和乳酸酪榭杆菌GG降低了H. pylori在AGS细胞和小鼠体内的粘附和由H. pylori感染造成的炎症。在所有益生菌中，乳酸菌酸杆菌NCFM和乳酸植物杆菌Lp-115在体外和体内对H. pylori的根除和炎症减轻产生显著效果。与H. pylori感染组相比，六种乳酸菌干预组的IL-8和TNF-α的mRNA和蛋白表达水平显著降低。每组1-7小时的尿素酶活性（ureA和ureB）变化表明，乳酸菌酸杆菌NCFM、乳酸菌酸杆菌La-14、乳酸植物杆菌Lp-115和乳酸酪榭杆菌GG有效降低了H. pylori的定殖。我们观察到，在H. pylori+NCFM+Lp-115小鼠体内，胃黏膜细胞外基质出现了更高比例的淋巴细胞和浆细胞浸润，中性粒细胞浸润增加。在H. pylori+NCFM+Lp-115组中，胃黏膜细胞外基质炎症细胞的浸润得到减少。此外，NCFM和Lp-115处理的C57BL/6小鼠中，IFN-γ的表达显著降低。乳酸菌酸杆菌NCFM和乳酸植物杆菌Lp-115可以降低H. pylori的粘附并抑制H. pylori感染引起的胃炎症反应。版权所有©2023 Shen, Ren, Qin, Bukhari, Yang, Gao, Ouwehand, Lehtinen, Zheng和Mi。

To determine the role of Lactobacillus strains and their combinations in inhibiting the colonization of H. pylori and gastric mucosa inflammation.Human gastric adenocarcinoma AGS cells were incubated with H. pylori and six probiotic strains (Lactobacillus acidophilus NCFM, L. acidophilus La-14, Lactiplantibacillus plantarum Lp-115, Lacticaseibacillus paracasei Lpc-37, Lacticaseibacillus rhamnosus Lr-32, and L. rhamnosus GG) and the adhesion ability of H. pylori in different combinations was evaluated by fluorescence microscopy and urease activity assay. Male C57BL/6 mice were randomly divided into five groups (uninfected, H. pylori, H. pylori+NCFM, H. pylori+Lp-115, and H. pylori+NCFM+Lp-115) and treated with two lactobacilli strains (NCFM and Lp-115) for six weeks. H. pylori colonization and tissue inflammation statuses were determined by rapid urease test, Hematoxylin-Eosin (HE) staining, immunohistochemistry, and qRT-PCR and ELISA.L. acidophilus NCFM, L. acidophilus La-14, L. plantarum Lp-115, L. paracasei Lpc-37, L. rhamnosus Lr-32, and L. rhamnosus GG reduced H. pylori adhesion and inflammation caused by H. pylori infection in AGS cells and mice. Among all probiotics L. acidophilus NCFM and L. plantarum, Lp-115 showed significant effects on the H. pylori eradication and reduction of inflammation in-vitro and in-vivo. Compared with the H. pylori infection group, the mRNA and protein expression levels of IL-8 and TNF-α in the six Lactobacillus intervention groups were significantly reduced. The changes in the urease activity (ureA and ureB) for 1-7h in each group showed that L. acidophilus NCFM, L. acidophilus La-14, L. plantarum Lp-115, and L. rhamnosus GG effectively reduced the colonization of H. pylori. We observed a higher ratio of lymphocyte and plasma cell infiltration into the lamina propria of the gastric mucosa and neutrophil infiltration in H. pylori+NCFM+Lp-115 mice. The infiltration of inflammatory cells in lamina propria of the gastric mucosa was reduced in the H. pylori+NCFM+Lp-115 group. Additionally, the expression of IFN-γ was decreased significantly in the NCFM and Lp-115 treated C57BL/6 mice.L. acidophilus NCFM and L. plantarum Lp-115 can reduce the adhesion of H. pylori and inhibit the gastric inflammatory response caused by H. pylori infection.Copyright © 2023 Shen, Ren, Qin, Bukhari, Yang, Gao, Ouwehand, Lehtinen, Zheng and Mi.