牙周炎是由牙周病菌引发的慢性炎症性疾病。研究表明，对细菌过度免疫反应参与了包括牙槽骨吸收在内的牙周组织破坏。Erucin是一种在欧芹等十字花科植物中发现的生物活性物质，属于异硫氰酸酯类化合物。以前的研究尚未尝试使用Erucin来治疗牙周炎，并且还没有一篇文章对Erucin对牙周居民细胞的作用进行过研究。本研究旨在分析Erucin对肿瘤坏死因子（TNF）-α刺激的TR146细胞，一种口腔上皮细胞系，产生的炎症和抗氧化介质的影响，包括其对信号分子的影响。我们的结果表明，Erucin可以抑制TNF-α刺激的TR146细胞中白细胞介素（IL）-6和CXC趋化因子配体（CXCL）10的产生以及血管细胞黏附分子（VCAM）-1的表达。此外，Erucin还诱导TR146细胞产生抗氧化酶血红素氧合酶（HO）-1和NAD（P）H喹诺酮脱氢酶（NQO）1。此外，Erucin还抑制了TNF-α刺激的TR146细胞中核因子（NF）-κB、信号转导与转录激活子（STAT）3和p70S6K-S6信号通路。我们已经证明，Erucin对口腔上皮细胞具有抗炎作用，并诱导抗氧化介质的产生。这些结果表明，Erucin可能成为一种新的用于牙周炎治疗的抗炎药物。

Periodontitis is a chronic inflammatory disease induced by periodontal disease-causing bacteria. It has been shown that excessive immune response against bacteria is involved in periodontal tissue destruction including alveolar bone resorption. Erucin is a biologically active substance found in cruciferous plants such as arugula, and is classified as an isothiocyanate. No previous studies have attempted to use erucin in the treatment of periodontitis, and there are no papers that have examined the effects of erucin on periodontal resident cells. The purpose of this study was to analyze the effects of erucin on the production of inflammatory and antioxidant mediators produced by tumor necrosis factor (TNF)-α-stimulated TR146 cells, an oral epithelial cell line, including its effects on signaling molecules. Our results indicate that erucin suppresses interleukin (IL)-6 and CXC-chemokine ligand (CXCL)10 production and vascular cell adhesion molecule (VCAM)-1 expression in TNF-α-stimulated TR146 cells. In addition, erucin induced the production of the antioxidant enzymes, Heme Oxygenase (HO)-1 and NAD(P)H quinone dehydrogenase (NQO)1 in TR146 cells. Furthermore, erucin suppressed TNF-α-stimulated nuclear factor (NF)-κB, signal transducer and activator of transcription (STAT)3, and p70S6K-S6 signaling pathways in TR146 cells. We have shown that erucin has anti-inflammatory effects on oral epithelial cells and also induces the production of antioxidant mediators. These results suggest that erucin may provide a new anti-inflammatory agent that can be used in the treatment of periodontitis.