除了2D细胞系和患者源异种移植模型外，器官样体作为一种替代性疾病模型，其体内生理相关性较好。然而，内源性和外源性限制都阻碍了这些器官样体的发展和临床转化。幸运的是，由于良好的生物相容性、出色的光学操控性和便利的化学修饰性，胶体光子晶体（PCs）已被应用于器官样体工程，并取得了有望重现细胞外基质微环境、具有定义明确的几何特征的初级培养、原位多生理参数监测、单细胞生物力学感知和用多功能数据输出进行高通量药物筛选的良好效果。在这里，我们回顾了由胶体光子晶体构建的器官样体工程领域的最新进展，并为未来的研究提供了参考意见。版权所有 © 2023. Elsevier B.V. 发表。

As alternative disease models, other than 2D cell lines and patient-derived xenografts, organoids have preferable in vivo physiological relevance. However, both endogenous and exogenous limitations impede the development and clinical translation of these organoids. Fortunately, colloidal photonic crystals (PCs), which benefit from favorable biocompatibility, brilliant optical manipulation, and facile chemical decoration, have been applied to the engineering of organoids and have achieved the desirable recapitulation of the ECM niche, well-defined geometrical onsets for initial culture, in situ multiphysiological parameter monitoring, single-cell biomechanical sensing, and high-throughput drug screening with versatile functional readouts. Herein, we review the latest progress in engineering organoids fabricated from colloidal PCs and provide inputs for future research.Copyright © 2023. Published by Elsevier B.V.