锌在水生物种的抗氧化能力和炎症反应中起着至关重要的作用，但其对大口鲈微鱼（Micropterus salmoides）的影响鲜有报道。因此，本文旨在研究不同水平锌对大口鲈微鱼生长性能、组织病理学、抗氧化能力和炎症细胞因子的影响。将初始体重为7.84±0.06 g的鱼类养殖10周。制备了添加蛋白质锌（比泊锌，Alltech）（0、30、60、90和120 mg/kg）的五个实验饲料，分别命名为Zn-42、Zn-73、Zn-103、Zn-133和Zn-164组。在饲料锌水平与存活率、整体鱼体粗脂肪含量或内脏体重指数之间没有明显差异。体重增加、体况因子、整体鱼体粗蛋白含量、白介素-10和转化生长因子-β基因表达水平在锌浓度达到102.68 mg/kg时逐渐增加，并在较高浓度下逐渐降低。肝脏肝脏指数、饲料转化率、肝脏中丙二醛水平、血清中天门冬氨酸转氨酶和丙氨酸转氨酶活性在锌浓度达到102.68 mg/kg时逐渐降低，并在此之上逐渐增加。核因子类似2血红蛋白衡样ECH相关蛋白1信号转导途径激活逐渐上调了肝脏谷胱甘肽过氧化物酶、总抗氧化能力、催化酶和超氧化物歧化酶的mRNA水平和活性，但该抗氧化能力在锌浓度超过102.68 mg/kg时较低。核因子-K-DNA结合和炎症细胞因子（白介素-1β、白介素-15、肿瘤坏死因子α和白介素-8）的基因表达水平在锌浓度达到102.68 mg/kg时上调，然后逐渐下调。总之，使用断线分析估计体重增加和蛋白质锌作为锌来源，建议大口鲈微鱼的膳食锌为66.57 mg/kg。版权所有 © 2023 Elsevier Ltd. 发表。

Zinc plays a crucial role in the antioxidant capacity, and inflammatory response of aquatic species, but its impact on largemouth bass Micropterus salmoides is rarely reported. Therefore, this paper aimed to investigate the effects of different levels of zinc on the growth performance, histopathology, antioxidant capacity, and inflammatory cytokines of largemouth bass Micropterus salmoides. Fish with an initial weight of 7.84 ± 0.06 g were cultured for 10 weeks. Five experimental diets were prepared with supplemented proteinate Zn (Bioplex Zn, Alltech) (0, 30, 60, 90, and 120 mg/kg), which were named the Zn-42, Zn-73, Zn-103, Zn-133, and Zn-164 groups. No evident difference was found between the dietary zinc level and the survival rate, the crude lipid content of the whole fish, or the visceral somatic index. Weight gain, condition factor, whole-body crude protein content, interleukin-10, and transforming growth factor beta gene expression were gradually enhanced with up to 102.68 mg/kg zinc and decreased at higher levels. The hepatosomatic index, feed conversion ratio, malondialdehyde level in the liver, aspartate aminotransferase, and alanine transaminase activity in the serum, gradually decreased up to 102.68 mg/kg zinc, and gradually increased beyond this. Activation of the nuclear factor erythroid-derived 2-like 2/Kelch-like ECH-associated protein 1 signaling pathway gradually up-regulated the mRNA levels and activities of glutathione peroxidase, total antioxidant capacity, catalase, and superoxide dismutase in the liver, this antioxidant ability was lower when the zinc was greater than 102.68 mg/kg. The gene expressions of nuclear factor-k-gene binding and pro-inflammation cytokines (interleukin-1β, interleukin-15, tumor necrosis factor alpha, and interleukin-8) were up-regulated up to 102.68 mg/kg zinc and then gradually repressed. In conclusion, using broken line analysis to estimate weight gain and Zn proteinate as the zinc source, the recommended dietary zinc for largemouth bass is 66.57 mg/kg zinc.Copyright © 2023. Published by Elsevier Ltd.