KCTD12钾通道四聚体化结构域12异常表达与各种肿瘤的发生和发展密切相关，但目前尚未进行关于KCTD12的全癌分析。我们利用癌症基因组图谱（TCGA）和基因表达数据库（GEO），探索了KCTD12与30多种人类恶性肿瘤之间的关联。首先，检查了KCTD12的mRNA和蛋白质水平，以及它们与肿瘤分期和生存率的相关性。其次，我们分析了CD8阳性和CD4阳性T细胞以及与癌症相关的成纤维细胞在肿瘤中的浸润，并探索了KCTD12表达与肿瘤细胞干细胞性、基因组异质性和诊断特异性之间的相关性。最后，我们利用KEGG/GO分析探索了与KCTD12相关的分子机制。结果显示，KCTD12的mRNA和蛋白质表达水平在大多数肿瘤中下降，与肿瘤患者的预后显著相关，KCTD12的磷酸化水平在胰腺腺癌（PAAD）、肺腺癌（LUAD）和乳腺浸润性癌（BRCA）等几种肿瘤中下降。KCTD12表达与宫颈鳞状细胞癌和子宫颈腺癌（CESC）、头颈鳞状细胞癌（HNSC）、PAAD和胃腺癌（STAD）中癌症相关成纤维细胞浸润程度呈正相关。KCTD12表达与CD8阳性和CD4阳性T细胞浸润之间的关系也得到了澄清。KCTD12对各种肿瘤具有较高的诊断敏感性，并可能通过影响肿瘤细胞干细胞性、肿瘤负担和其他特征参与肿瘤细胞生物学。最后，我们分析了KCTD12的分子功能和可能的KEGG/GO信号通路。在本研究中，我们开发了一个用于全癌的诊断、预后和免疫浸润的生物标志物。© 2023. Springer Nature Limited.

Abnormal expression of the potassium channel tetramerization domain containing 12 (KCTD12) is closely related to the occurrence and development of various tumors, but a pan-cancer analysis of KCTD12 has not yet been conducted. We explored the association between KCTD12 and more than 30 human malignancies using The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. First, the mRNA and protein levels of KCTD12 were examined and their correlations with tumor stage and survival were explored. Second, we analyzed the infiltration of CD8+ and CD4+ T cells and cancer-associated fibroblasts in tumors and explored the correlation between KCTD12 expression and tumor cell stemness, genomic heterogeneity, and diagnostic specificity. Finally, we explored the molecular mechanisms associated with KCTD12 using KEGG/GO analysis. The results showed that KCTD12 mRNA and protein expression levels decreased in most tumors was significantly associated with the prognosis of tumor patients, and the phosphorylation level of KCTD12 decreased in several tumors, such as S200 and T196, pancreatic adenocarcinoma (PAAD), lung adenocarcinoma (LUAD), and breast invasive cancer (BRCA). The expression of KCTD12 was positively correlated with the degree of cancer-associated fibroblasts infiltration in cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC), head and neck squamous cell carcinoma (HNSC), PAAD, and stomach adenocarcinoma (STAD). The relationship between KCTD12 expression and CD8+ and CD4+ T cell infiltration was also clarified. KCTD12 showed high diagnostic sensitivity for various types of tumors and may be involved in tumor cell biology by affecting tumor cell stemness, tumor burden, and other characteristics. Finally, we analyzed the molecular functions of KCTD12 and possible KEGG/GO signaling pathways. In this study, we developed a biological marker for diagnosis, prognosis, and immune infiltration of the pan-cancers.© 2023. Springer Nature Limited.