绿色生物合成纳米颗粒具有广阔的前景，因为它们可以通过一种比物理或化学合成更能节能、经济、可重复和环境友好的方法来生产。本研究使用福士德真菌隔离AJTYC1产生了银纳米颗粒（AgNPs）。采用多种技术对合成的AgNPs进行表征，包括UV-Vis光谱、透射电子显微镜、Zeta电位分析、X射线衍射分析、能谱分析和傅里叶变换红外光谱。AgNPs在310 nm处呈现出明显的表面等离子共振（SPR）峰。生物合成的AgNPs形态为球状，透射电子显微镜图像显示其尺寸在27.3至53.1 nm之间。FT-IR结果的显著峰表明了烷烃、炔烃、环烯烃、羧酸、芳香胺、酯和酚类化合物的不同基团。此外，结果表明AgNPs在抗氧化活性方面优于抗坏血酸和叔丁基羟基甲苯（一种强抗氧化剂）。此外，AgNPs通过琼脂扩散法对革兰氏阳性菌、革兰氏阴性菌和抗真菌活性也表现出良好效果。AgNPs的抗癌活性因其所用于治疗的癌症类型而异，包括肝细胞癌（HepG2）、结肠直肠癌（HCT116）和乳腺癌（MCF7）。随着AgNP浓度的增加，癌细胞系的存活率降低。AgNPs还通过将亚甲基蓝、孔雀石绿、靛青和绿色孔雀石白降解分别达到了88.3%、81.5%、76.4%和78.2%的有希望的光催化活性。此外，与对照相比，AgNPs对洋葱（Allium cepa）植物的有丝分裂指数和染色体异常产生了显著影响。因此，合成的AgNPs展示了一种高效、环保和可持续的方法，用于脱色染料以及抗氧化、抗菌、抗真菌和抗癌活性。这可能是在抗击多种动态疾病和减少废水染料污染方面取得的重大胜利。© 2023. The Author(s).

Green biosynthesized nanoparticles have a bright future because they can be produced using a method that is more energy-efficient, cost-effective, repeatable, and environmentally friendly than physical or chemical synthesis. In this study, silver nanoparticles (AgNPs) were produced using the Fusarium nygamai isolate AJTYC1. Several techniques were used to characterize the synthesized AgNPs, including UV-Vis spectroscopy, transmission electron microscope, zeta potential analysis, X-ray diffraction analysis, energy dispersive X-ray, and Fourier transform-infrared spectroscopy. AgNPs showed a distinctive surface plasmon resonance (SPR) peak in the UV-visible range at 310 nm. The morphology of the biosynthesized AgNPs was spherical, and the TEM image shows that they ranged in size from 27.3 to 53.1 nm. The notable peaks of the FT-IR results show the different groups for the alkane, alkynes, cyclic alkenes, carboxylic, aromatic amine, esters, and phenolics. Additionally, the results showed that AgNPs had superior antioxidant activity when compared to ascorbic acid and butylated hydroxytoluene, which is a powerful antioxidant. Additionally, AgNPs have antibacterial action utilizing agar diffusion against gram-positive bacteria, gram-negative bacteria, and antifungal activity. AgNPs' anticancer activity varied depending on the type of cancer it was used to treat, including hepatocellular cancer (HepG2), colorectal carcinoma (HCT116), and breast cancer of the mammary gland (MCF7). The viability of the cancer cell lines was reduced with increasing AgNP concentration. AgNPs also demonstrated promising photocatalytic activity by reducing methylene blue, safranin, crystal violet, and green malachite by 88.3%, 81.5%, 76.4%, and 78.2%, respectively. In addition, AgNPs significantly affected the Allium cepa plant's mitotic index and resulted in chromosomal abnormalities as compared to the control. Thus, the synthesized AgNPs demonstrated an efficient, eco-friendly, and sustainable method for decolorizing dyes as well as antioxidant, antibacterial, antifungal, and anticancer activities. This could be a huge victory in the fight against numerous dynamic diseases and lessen wastewater dye contamination.© 2023. The Author(s).