对于未加工、天然和更健康的食品产品的需求不断增长，促使人们寻找替代的、多功能的生物活性食品成分。因此，本研究重点研究了来自酿酒酵母代谢的一种肽分离物的功能属性。对肽分离物的抗菌活性进行了评估，包括针对多种引起食物中毒的病原体，如白色念珠菌、卡鲁斯氏念珠菌、大肠杆菌、单核细胞增生李斯特菌和沙门菌等。使用FRAP和DPPH清除能力测定法评估了肽分离物的抗氧化性能。此外，利用结肠癌细胞和正常结肠上皮细胞评估了肽分离物的细胞毒性，并通过α-淀粉酶和α-葡萄糖苷酶抑制测定分析其作为抗糖尿病药物的潜力。结果表明，2-10千达肽分离物对所有测试的微生物，除了卡鲁斯氏念珠菌具有抗菌作用。大肠杆菌、单核细胞增生李斯特菌和沙门菌的最小抑制浓度保持在0.25毫克/毫升，而白色念珠菌需要更高的浓度1.0毫克/毫升。此外，肽分离物表现出抗氧化活性，表现为DPPH自由基清除活性为81.03％，FRAP值为1042.50 ± 32.5微摩尔TE/毫升(在1.0毫克/毫升时)。肽分离物在肿瘤和非肿瘤人类细胞中的浓度高达0.3毫克/毫升时不显示细胞毒性。此外，肽分离物表现出抗炎活性，显著降低了未刺激的结肠细胞中TNFα基因的表达超过29.7％，并在脂多糖刺激的结肠细胞中降低50％。它还抑制了碳水化合物消化酶α-淀粉酶(IC50为199.3 ± 0.9微克/毫升)和α-葡萄糖苷酶(IC20为270.6 ± 6.0微克/毫升)的活性。总的来说，研究结果显示肽分离物具有抗菌、抗氧化、抗炎和抗糖尿病活性。该研究在评估酿酒酵母生物活性肽的功能生物学性质方面迈出了一步。

The rising demand for minimally processed, natural, and healthier food products has led to the search for alternative and multifunctional bioactive food components. Therefore, the present study focuses on the functional proprieties of a peptide fraction derived from Saccharomyces cerevisiae metabolism. The antimicrobial activity of the peptide fraction is evaluated against various foodborne pathogens, including Candida albicans, Candida krusei, Escherichia coli, Listeria monocytogenes, and Salmonella sp. The peptide fraction antioxidant properties are assessed using FRAP and DPPH scavenging capacity assays. Furthermore, the peptide fraction's cytotoxicity is evaluated in colorectal carcinoma and normal colon epithelial cells while its potential as an antidiabetic agent is investigated through α-amylase and α-glucosidase inhibitory assays. The results demonstrate that the 2-10 kDa peptide fraction exhibits antimicrobial effects against all tested microorganisms, except C. krusei. The minimal inhibitory concentration for E. coli, L. monocytogenes, and Salmonella sp. remains consistently low, at 0.25 mg/mL, while C. albicans requires a higher concentration of 1.0 mg/mL. Furthermore, the peptide fraction displays antioxidant activity, as evidenced by DPPH radical scavenging activity of 81.03%, and FRAP values of 1042.50 ± 32.5 µM TE/mL at 1.0 mg/mL. The peptide fraction exhibits no cytotoxicity in both tumor and non-tumoral human cells at a concentration up to 0.3 mg/mL. Moreover, the peptide fraction presents anti-inflammatory activity, significantly reducing the expression of the TNFα gene by more than 29.7% in non-stimulated colon cells and by 50% in lipopolysaccharide-stimulated colon cells. It also inhibits the activity of the carbohydrate digestive enzymes α-amylase (IC50 of 199.3 ± 0.9 µg/mL) and α-glucosidase (IC20 of 270.6 ± 6.0 µg/mL). Overall, the findings showed that the peptide fraction exhibits antibacterial, antioxidant, anti-inflammatory, and antidiabetic activity. This study represents a step forward in the evaluation of the functional biological properties of S. cerevisiae bioactive peptides.