Cryptosporidiosis是一种在人类和动物中由Cryptosporidium的孢子感染引起的人兽共患病。微小RNA（miRNA）是调节寄生虫感染的先天免疫反应的重要因子。公共miRNA数据用于研究真实宿主中cryptosporidiosis致病机制是非常有限的。在本研究中，我们使用微阵列测序比较了C. muris感染和未感染的BALB/c小鼠的腺胃中的miRNA谱。在BALB/c小鼠感染C. muris 8小时后，共鉴定出10种miRNA（包括3种上调和7种下调的miRNA），具有显著差异表达（|FC| ≥ 2且P值 <  0.05）。使用MiRWalk和miRDB在线生物信息学工具预测了差异表达miRNA的靶基因。进行基因本体论（GO）和基因组学百科全书（KEGG）富集分析进行靶基因注释。GO分析表明与基因转录和离子传输相关的GO术语有显著富集。此外，KEGG分析显示目标基因与不同类型的肿瘤疾病进展和抗病原体免疫途径密切相关。在当前研究中，我们首次报告了C. muris侵袭的早期阶段BALB/c小鼠腺胃中的miRNA表达谱变化。miRNA表达的这种失调可能有助于我们对cryptosporidiosis病理学的理解。该研究提供了对cryptosporidiosis miRNA调控机制的新视角，可能有助于制定针对该病原体的有效控制策略的发展。版权所有 © 2023 Elsevier Inc.发布。

Cryptosporidiosis is a zoonotic disease in humans and animals that is caused by infection with the oocysts of Cryptosporidium. MicroRNAs (miRNAs) are important players in regulating the innate immune response against parasitic infection. Public miRNAs data for studying pathogenic mechanisms of cryptosporidiosis, particularly in natural hosts, are scarce. Here, we compared miRNA profiles of the glandular stomach of C. muris-infected and uninfected BALB/c mice using microarray sequencing. A total of 10 miRNAs (including 3 upregulated and 7 downregulated miRNAs) with significant differential expression (|FC| ≥ 2 and P value < 0.05) were identified in the glandular stomach of BALB/c mice 8 h after infection with C. muris. MiRWalk and miRDB online bioinformatics tools were used to predict the target genes of differentially expressed miRNAs. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed to annotate the target genes. GO analysis indicate that gene transcription-related and ion transport-related GO terms were significantly enriched. In addition, the KEGG analyses showed that the target genes were strongly related to diverse types of tumor disease progression and anti-pathogen immunity pathways. In the current study, we firstly report changes in miRNA expression profiles in the glandular stomach of BALB/c mice at the early phase of C. muris invasion. This dysregulation in miRNA expression may contribute to our understanding of cryptosporidiosis pathology. This study provides a new perspective on the miRNA regulatory mechanisms of cryptosporidiosis, which may help in the development of effective control strategies against this pathogen.Copyright © 2023. Published by Elsevier Inc.