多柔比星（Dox）可能导致卵泡的丧失，进而耗尽卵巢储备，导致早发性卵巢功能衰竭。硒（Se）是一种具有基本生物特性的微量元素，也是最常见的化学抑制剂化合物之一。本研究描述了不同硒剂量饮食补充对Dox诱导的大鼠卵巢损伤的治疗作用。本研究将64只成年雌性Wistar大鼠随机分为八组：对照组、Dox组（5mg/kg腹腔注射[i.p.]）、低剂量硒组（0.5mg/kg i.p.）、中剂量硒组（1mg/kg i.p.）、高剂量硒组（2mg/kg i.p.）、Dox+低剂量硒组（0.5mg/kg i.p.）、Dox+中剂量硒组（1mg/kg i.p.）和Dox+高剂量硒组（2mg/kg i.p.）。实验结束后，统计卵泡数，并测定抗米勒尔素、白细胞介素1 β、肿瘤坏死因子α和半胱氨酸蛋白酶3的表达。在卵巢组织中，生物化学测量马术面丙二醛、超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的水平。Dox导致卵巢损伤，表现为卵巢标志物的显著变化、组织学异常以及抗氧化防御机制的衰弱。此外，Dox治疗显著改变了炎症和凋亡标志物的表达。研究了注射1mg硒与不同饱和度的Dox，结果显示了组织病理学和卵泡储备的保护特性。1mg硒预处理通过缓解抗氧化机制、减少炎症和凋亡以及恢复卵巢结构，改善Dox诱导的卵巢毒性。因此，我们的研究结果表明，1mg硒是预防与Dox相关的卵巢损伤的一种有前景的治疗药物。© 2023 Wiley Periodicals LLC.

Doxorubicin (Dox) may induce loss of follicles, resulting in the depletion of ovarian reserve and consequent premature ovarian failure. Selenium (Se) is an oligoelement with fundamental biological features and is among the most common chemical inhibitor compounds. The present study describes the curative effects of dietary supplementation with different Se doses on Dox-induced ovarian damage in rats. In this study, 64 adult female Wistar rats were randomly separated into eight groups: Control group, Dox group (5 mg/kg intraperitoneal [i.p.]), low-dose Se (0.5 mg/kg i.p.), middle dose Se (1 mg/kg i.p.), high dose (Se 2 mg/kg i.p.), Dox + low-dose Se group (0.5 mg/kg i.p.), Dox + middle dose Se (1 mg/kg i.p.), and Dox + high-dose Se group (2 mg/kg i.p.). After the experiment, ovarian follicles were counted, and Antimüllerian hormone, interleukin 1 beta, tumor necrosis factor alpha, and caspase-3 expression were determined. Levels of malondialdehyde, superoxide dismutase, catalase, and glutathione peroxidase were biochemically measured in ovarian tissue. Dox caused ovarian injury, as evidenced by significant changes in ovarian markers, histological abnormalities, and the debilitation of antioxidant defense mechanisms. Furthermore, Dox therapy significantly changed the expression of inflammatory and apoptotic markers. Dox + 1 mg Se with various saturations was studied, and this study demonstrated both histopathological and follicular reserve and more protective features. 1 mg Se pretreatment improved Dox-induced ovarian toxicity through alleviating the antioxidant mechanism, decreasing inflammation and apoptosis, and restoring ovarian architecture. As a result, our findings indicate that 1 mg Se is a promising therapeutic agent for the prevention of ovarian damage associated with Dox.© 2023 Wiley Periodicals LLC.