“表观遗传学”概念首次于1942年被描述。迄今为止，组蛋白、DNA和RNA上的化学修饰已经被发现成为表观遗传学修饰的三个重要构件。许多表观遗传修饰已经被广泛研究，并发现其参与了大多数重要的生物过程以及人类疾病，包括癌症。准确、定量地绘制超过100种已知的组蛋白修饰、17种已知的DNA修饰和160种已知的RNA修饰是理解表观遗传修饰在多样生物过程基因调控中的作用的关键。随着测序技术的快速发展，科学家能够使用各种定量、高分辨率、全基因组/转录组的方法检测特定的表观遗传修饰。在这里，我们总结了哺乳动物细胞中主要的组蛋白、DNA和RNA修饰的表观遗传修饰测序技术的最新进展。©2023. 作者。

The "epigenetics" concept was first described in 1942. Thus far, chemical modifications on histones, DNA, and RNA have emerged as three important building blocks of epigenetic modifications. Many epigenetic modifications have been intensively studied and found to be involved in most essential biological processes as well as human diseases, including cancer. Precisely and quantitatively mapping over 100 [1], 17 [2], and 160 [3] different known types of epigenetic modifications in histone, DNA, and RNA is the key to understanding the role of epigenetic modifications in gene regulation in diverse biological processes. With the rapid development of sequencing technologies, scientists are able to detect specific epigenetic modifications with various quantitative, high-resolution, whole-genome/transcriptome approaches. Here, we summarize recent advances in epigenetic modification sequencing technologies, focusing on major histone, DNA, and RNA modifications in mammalian cells.© 2023. The Author(s).