细胞外囊泡（类似外泌体的囊泡）是由各种细胞释放到细胞外空间的大小为20-200nm的小型膜囊泡。这些细胞外囊泡在细胞间的通信中起着重要作用，包含蛋白质、mRNA、microRNA（miRNA）和长非编码RNA（lncRNA）等物质。来自侵袭性结肠癌细胞系的外泌体对血管生成的影响尚不清楚。因此，本研究的目的是研究来自侵袭性结肠癌细胞系的外泌体对内皮细胞血管生成的影响。 在本研究中，对侵袭性结肠癌细胞系SW480-7的细胞培养上清液中的外泌体进行了表征。在与SW480-7分泌的外泌体共培养后，对微血管内皮细胞（用端粒酶免疫修饰的微血管内皮细胞，简称TIME）的管状结构形成和血管生成相关基因的表达进行了检查。Zetasizer结果显示，来自SW480-7的外泌体的平均直径为246.2nm，并且形态分析显示大多数外泌体的大小小于200nm。结果表明，来自SW480-7的外泌体增加了TIME的管状结构形成，并上调了FGFR3 mRNA在TIME中的表达。 我们的研究结果表明，来自SW480-7的外泌体增加了TIME的管状结构形成，并上调了FGFR3 mRNA在TIME中的表达。

Extracellular vesicles (exosome-like vesicles) are small membrane vesicles ranging from 20-200nm in size that are released by various cells into the extracellular space. These extracellular vesicles play a major role in cell-to-cell communication and contain materials, such as proteins, mRNAs, microRNAs (miRNAs) and long non-coding RNAs (lncRNAs). The effect of exosomes derived from an invasive colon cancer cell line on angiogenesis is unclear. Hence, the aim of this study is to investigate the effect of exosomes derived from an invasive colon cancer cell line on angiogenesis of endothelial cells.In the present study, the exosomes from the cell culture supernatants of an invasive colon cancer cell line SW480-7 were characterised. The effect on tube formation and expression of angiogenic genes in a microvascular endothelial cell, telomerase-immortalised microvascular endothelial cell (TIME) was examined after co-cultured with exosomes secreted from SW480-7.Zetasizer result showed average diameter of exosomes derived from SW480-7 was 246.2 nm and morphological analysis showed the size of majority of exosomes were less than 200 nm. Results showed that exosomes derived from SW480-7 increased tube formation and up-regulated FGFR3 mRNA expression in TIME.Our findings suggest that exosomes derived from SW480-7 increased tube formation and up-regulated expression of FGFR3 mRNA in TIME.