miRNAs在三重阴性乳腺癌（TNBC）中作为潜在的生物标志物发挥着关键作用。在此，成功构建了一种基于电化学发光共振能量转移（ECL-RET）的高效信号"打开-关闭"型生物传感器，用于TNBC中miRNA-150-5p的测定。ECL-RET调控的传感平台由NiMn-LDH纳米花、人工组装的磷脂双层和标记有发光Eu掺杂MoS2量子点的发夹DNA组成。首先，制备了具有高量子效率的发光Eu掺杂MoS2量子点作为ECL-RET供体。然后，利用广泛的紫外-可见吸收光谱的NiMn双层氢氧化物（LDH）纳米花作为ECL-RET受体。其次，由于发夹DNA的结构，ECL-RET供-受体对之间的紧密距离可以熄灭Eu掺杂MoS2量子点的发光信号。当miRNA-150-5p被捕获时，发夹DNA结构发生变化，变成了杆状构型，使Eu掺杂MoS2量子点与NiMn-LDH之间的距离增加。因此，可以观察到ECL信号的恢复，呈现出信号"打开-关闭"的模式。此外，磷脂双层的亲水性可以减少非特异性吸附，并有效提高发夹DNA的灵活性。因此，基于ECL-RET调控策略，该生物传感器能够在10 fM至1 nM范围内检测miRNA-150-5p，检测限为1.5 fM。构建的生物传感器可以有效区分TNBC患者肿瘤和健康乳腺纤维腺瘤。ECL-RET调控策略为超灵敏生物分子检测提供了一条新的途径，并促进了TNBC的诊断和治疗的发展。版权所有 © 2023 Elsevier B.V.。保留所有权利。

MiRNAs played critical roles in triple negative breast cancer (TNBC) as potential biomarkers. Herein, an efficient signal "off-on" mode-biosensor based on electrochemiluminescence resonance energy transfer (ECL-RET) was successfully constructed for the miRNA-150-5p determination in TNBC. The ECL-RET regulated-sensing platform consisted of NiMn-LDHs nanoflowers, the artificially assembled phospholipid bilayers and hairpin DNA-labeled Eu-doped MoS2 QDs. Firstly, Eu-doped MoS2 QDs with high quantum efficiency were prepared as the ECL-RET donors. And NiMn-layer double hydroxides (LDHs) nanoflowers with wide UV-vis absorption spectra as the ECL-RET acceptors. Secondly, due to the hairpin DNA structure, the closed distance between ECL-RET donor-acceptor pair can quench the luminescence signal of Eu-doped MoS2 QDs. When miRNA-150-5p was captured, the hairpin DNA structure changed to a rodlike configuration and enlarged the distance between Eu-doped MoS2 QDs and NiMn-LDHs. As a result, the recovery of ECL signal can be observed as a signal "turn off-on" mode. Furthermore, the hydrophilicity of the lipid bilayer can reduce the nonspecific adsorption and improve the flexibility of the hairpin DNA efficiently. Therefore, based on the ECL-RET regulation strategy, the biosensor was employed to detect miRNA-150-5p from 10 fM to 1 nM with a detection limit of 1.5 fM. The constructed biosensor can effectively differentiate TNBC patient tumor and healthy breast fibroadenoma. The ECL-RET regulation strategy provided a new biosensing pathway for ultrasensitive detection of biomolecules and promoted the development of diagnosis and treatment of TNBC.Copyright © 2023 Elsevier B.V. All rights reserved.