2020年全球新诊断的第四常见癌症和导致女性癌症死亡的第四大原因是宫颈癌，预计有604,000例新病例和342,000例死亡病例。其复发和转移率高。发现新的靶点可能有助于预测和治疗宫颈癌。NADPH氧化酶1(NOX1)基因介导的活性氧(ROS)产生可能诱导宫颈癌细胞的迁移和侵袭。肿瘤相关巨噬细胞(TAMs)在宫颈癌中发挥重要作用。肿瘤细胞来源的外泌体介导肿瘤与肿瘤微环境之间的信号传导。阐明NOX1携带外泌体在调节TAMs中所涉及的机制可能为宫颈癌的进展提供有价值的见解。从UCSC数据库下载了统一标准化的全癌症mRNA数据。使用R语言软件计算了每种肿瘤类型的肿瘤和邻近正常组织中NOX1的表达，并进行了显著差异分析。使用MuTect2软件从GDC下载了所有TCGA样本的SNP数据集。使用细胞实验和动物肿瘤形成实验评估了外泌体NOX1是否通过刺激ROS产生来促进宫颈癌中TAM的M2极化。NOX1在全癌中高度表达，突变频率较低。NOX1的上调可能与宫颈癌组织中M2型巨噬细胞的浸润相关，NOX1通过刺激ROS产生促进了宫颈癌细胞的恶性特征。外泌体NOX1通过刺激ROS产生促进TAM M2极化。外泌体NOX1通过刺激ROS产生促进了宫颈癌和M2极化的进展。外泌体NOX1通过刺激ROS产生促进TAM M2极化介导宫颈癌的癌症进展。© 2023. BioMed Central Ltd., Springer Nature的一部分。

Cervical cancer the fourth most frequently diagnosed cancer and the fourth leading cause of cancer death in women, with an estimated 604,000 new cases and 342,000 deaths worldwide in 2020 for high rates of recurrence and metastasis. Identification of novel targets could aid in the prediction and treatment of cervical cancer. NADPH oxidase 1 (NOX1) gene-mediated production of reactive oxygen species (ROS) could induce migration and invasion of cervical cancer cells. Tumor-associated macrophages (TAMs) play important roles in cervical cancer. Tumor cell-derived exosomes mediate signal transduction between the tumor and tumor microenvironment. Elucidation of the mechanisms of NOX1-carrying exosomes involved in the regulation of TAMs may provide valuable insights into the progression of cervical cancer.Uniformly standardized mRNA data of pan-carcinoma from the UCSC database were downloaded. Expression of NOX1 in tumor and adjacent normal tissues for each tumor type was calculated using R language software and significant differences were analyzed. SNP data set were downloaded for all TCGA samples processed using MuTect2 software from GDC. Cell experiment and animal tumor formation experiment were used to evaluate whether exosomal NOX1 stimulating ROS production to promote M2 polarization of TAM in cervical cancer.NOX1 is highly expressed with a low mutational frequency in pan-carcinoma. Upregulation of NOX1 may be associated with infiltration of M2-type macrophages in cervical cancer tissues, and NOX1 promotes malignant features of cervical cancer cells by stimulating ROS production. Exosomal NOX1 promotes M2 polarization of by stimulating ROS production. Exosomal NOX1 enhances progression of cervical cancer and M2 polarization in vivo by stimulating ROS production.Exosomal NOX1 promotes TAM M2 polarization-mediated cancer progression through stimulating ROS production in cervical cancer.© 2023. BioMed Central Ltd., part of Springer Nature.