肿瘤生长因子-β（TGF-β）是一种在各种生物过程中发挥关键作用的多功能细胞因子。TGF-β还参与各种病理过程，包括乳腺癌（BC）。BC严重依赖激素受体，如雌激素受体（ERα，ERβ）和孕激素受体（PR）。为了评估TGF-β与激素受体（ER和PR）的分子分布之间的可能相互作用。本研究通过qRT-PCR分析了40个乳腺肿瘤组织中SMAD3，ERα，ERβ和PR的表达模式。此外，通过免疫组织化学检测了Ki-67和HER2/neu状态。 我们的结果显示，在40例检测中，27例SMAD3表达下降，而其表达上升的13例（p=0.003）。SMAD3的过表达与高等级肿瘤相关。此外，SMAD3与高增殖指数和转移存在显著正相关（p=0.001和p=0.01）。SMAD3与（ERα，ERβ）亚组的表达相对于（ERα，ERβ）亚组的显著关联（p=0.009）。对于PR来说也是如此，我们的结果显示SMAD3与PR的显著相关（p=0.02）。此外，与临床和病理信息相比，（SMAD3+，ERα+，ERβ+）及（SMAD3+，PR+）分子亚组的表达分析显示与高级别肿瘤、高增殖指数（p=0.02，p=0.01）和淋巴结浸润存在显著关联。 综上所述，SMAD3可能在（ERα+，ERβ+）和PR+乳腺癌中促进细胞增殖和转移。

Tumor Growth Factor-β (TGF-β) is a multifunctional cytokine that plays a crucial role in various biological processes. TGF-β is also involved in various pathologies including breast cancer (BC). BC is strongly dependent on hormone receptors such as Estrogen receptors (ERa, ERb) and Progesterone Receptor (PR).To audit the potential cross-talk between TGF-β and the molecular distribution of hormone receptors (ERs and PR).The current study analyzes the expression patterns of SMAD3, ERα, ERβ and PR in 40 breast tumor tissues using qRT-PCR. Furthermore, the Ki-67 and HER2/neu status have been detected by Immunohistochemistry.Our results show a decrease in the SMAD3 expression in 27 of the 40 cases while its expression is increased in the remaining 13 cases (p=0.003). The over-expression of SMAD3 is associated with high tumor grades. Moreover, there is a significant positive correlation between SMAD3+ with a high proliferative index and metastases (p=0.001 and p=0.01respectevely). The SMAD3 expression relative to (ERα, ERβ) subgroups shows a significant association of SMAD3+ with the (ERα+, ERβ+) subgroups (p=0.009). The same is true for PR, our results show a significant association of SMAD3+ with PR+ (p=0.02). Moreover, analysis of the expression of molecular subgroups (SMAD3+, ERα+, ERβ+) and (SMAD3+, PR+) compared to clinical and pathological information shows a significant association with high grade tumors, a high proliferation index (p=0.02, p= 0.01 respectively) and lymph node infiltration.It is concluded that SMAD3 can promote cell proliferation and metastases in (ERα+, ERβ+) and PR+ breast cancer.