子宫颈最小偏移腺癌（MDA），也称为恶性腺瘤，是一种罕见的子宫颈腺癌亚型，其组织学特征类似于良性肿瘤，导致诊断率低和缺乏有效治疗方法。MDA在单细胞分辨率下及肿瘤微环境（TME）中的转录组特征尚不清楚。在本研究中，我们对MDA样本（Ca）和相邻的正常组织（PCa）进行了单细胞转录组分析。本研究揭示了乳腺导管腺癌（MDA）样本中树突状细胞（DCs）和T细胞的普遍存在，DCs经历了显著的代谢重编程和免疫应激。此外，我们的研究结果显示了DCs和T细胞在MDA发病和转移进展中的关键参与，这得到了对MDA和HPV样本的单细胞转录组谱分析的证实。这一资源更深入地了解MDA的慢性特性，可能有助于MDA免疫治疗的开发。版权所有© 2023 Elsevier B.V. 保留所有权利。

Minimal deviation adenocarcinoma (MDA) of the uterine cervix, also referred to as malignant adenoma, is a rare subtype of cervical adenocarcinoma that exhibits histological characteristics resembling those of benign tumors, resulting in a low diagnostic rate and a lack of effective treatment options. The transcriptomic features of MDA at the single-cell resolution and within the tumor microenvironment (TME) remain unclear. In this study, we conducted single-cell transcriptomic analyses of MDA samples (Ca) and adjacent normal tissues (PCa). The present study reveals the prevalence of dendritic cells (DCs) and T cells in the carcinoma (Ca) of mammary ductal adenocarcinoma (MDA), with DCs undergoing significant metabolic reprogramming and immune stress. Additionally, our findings demonstrate the crucial involvement of DCs and T cells in the pathogenesis and metastatic progression of MDA, as evidenced by single-cell transcriptomic profiling of MDA and HPV samples. This resource provides a more profound understanding of the indolent nature of MDA and may prove useful in the development of MDA immunotherapy.Copyright © 2023 Elsevier B.V. All rights reserved.