将成像、诊断和治疗功能融合到单一试剂中的多功能纳米平台对疾病，特别是肿瘤的早期诊断和高效治疗具有重要意义。本研究报告了一种新型的石墨烯包裹的花瓣状间隙增强的拉曼标签，其具有介孔二氧化硅外壳（MS-GP-GERTs）。这些 MS-GP-GERTs 在金纳米核心和花瓣状外壳之间的间隙中嵌有 4-NBT 拉曼探针，并被石墨烯和介孔二氧化硅包裹。光热测量实验结果表明，石墨烯层显著增强了间隙增强的拉曼标签（GERTs）的光热效应。MS-GP-GERTs 的光热转换效率达到 40.8%，与纯石墨烯相当。此外，MS-GP-GERTs 在琼脂模型中表现出良好的光热性能，在低功率密度激光（0.5 W/cm2）下，在 5 分钟内将模型加热至 47°C。MS-GP-GERTs 还表现出优异的光热稳定性和生理环境稳定性，成为重复光热治疗的有前景的候选试剂。拉曼光谱和成像实验显示，MS-GP-GERTs 具有较低的检测限（100 fM），较大的成像深度（2.74 mm），并且具备在仿真生物组织和细胞中成像的优良能力。这种新型拉曼标签具有成为集成拉曼成像诊断和光热治疗的多功能纳米平台的潜力。 版权所有 © 2023 Elsevier B.V. 保留所有权利。

Multifunctional nanoplatform that combine imaging, diagnostic, and therapeutic functions into a single agent have great significance for the early diagnosis and efficient treatment of diseases, particularly tumors. In this study, we report on a novel graphene-wrapped petal-like gap-enhanced Raman tags with mesoporous silica shells (MS-GP-GERTs). These MS-GP-GERTs have 4-NBT Raman reporters embedded in the gap between the gold nanocore and the petal-shaped shell and are wrapped in graphene and mesoporous silica. The results of photothermal measurement experiments show that graphene layers significantly enhanced the photothermal effect of gap-enhanced Raman tags (GERTs). The photothermal conversion efficiency of MS-GP-GERTs reaches 40.8%, comparable to pure graphene. Moreover, MS-GP-GERTs show good photothermal performance in agarose phantoms, heating the phantom to 47 °C within 5 min under a low power density laser (0.5 W/cm2). MS-GP-GERTs also exhibit excellent photothermal stability and physiological environment stability, making them a promising candidate for repeated photothermal therapy. Raman spectra and mapping imaging experiments demonstrate MS-GP-GERTs' low detection limit (100 fM), large imaging depth (2.74 mm), and excellent ability to image simulated biological tissue and cells. This novel Raman tag has the potential to become a multifunctional nano platform for integrating Raman imaging diagnosis and photothermal therapy.Copyright © 2023 Elsevier B.V. All rights reserved.