海洋天然化合物（Neviotin A）通过引发转录组变化和细胞死亡，在MCF-7细胞中展示了抗癌功效。
Marine Natural Compound (Neviotin A) Displays Anticancer Efficacy by Triggering Transcriptomic Alterations and Cell Death in MCF-7 Cells.
发表日期：2023 Aug 28
作者：
Quaiser Saquib, Stefan Schwaiger, Mostafa Alilou, Sarfaraz Ahmed, Maqsood A Siddiqui, Javed Ahmad, Mohammad Faisal, Eslam M Abdel-Salam, Rizwan Wahab, Adnan J Al-Rehaily, Hermann Stuppner, Abdulaziz A Al-Khedhairy
来源：
Cell Death & Disease
摘要：
本研究探究了海绵（Haliclona fascigera）氯仿萃取物（样品C）在人类乳腺腺癌（MCF-7）细胞中的抗癌机制。通过MTT和中性红摄取（NRU）实验对细胞存活率进行分析，结果显示样品C处理降低了细胞的增殖能力。流式细胞术数据展示了样品C处理的MCF-7细胞中反应性氧化物（ROS）、一氧化氮（NO）、线粒体电位功能障碍和细胞凋亡。样品C处理的MCF-7细胞的qPCR阵列显示了不同凋亡通路之间的相互作用，特别是BIRC5、BCL2L2和TNFRSF1A基因。免疫荧光分析证实了裂解细胞中p53、bax、bcl2、MAPKPK2、PARP-1和caspase-3蛋白的定位。样品C的生物引导分馏揭示了Neviotin A作为活性物质，可在MCF-7中引发最大的细胞死亡，说明其作为治疗人类乳腺癌药物开发的药理学效力。
We investigated the anticancer mechanism of a chloroform extract of marine sponge (Haliclona fascigera) (sample C) in human breast adenocarcinoma (MCF-7) cells. Viability analysis using MTT and neutral red uptake (NRU) assays showed that sample C exposure decreased the proliferation of cells. Flow cytometric data exhibited reactive oxygen species (ROS), nitric oxide (NO), dysfunction of mitochondrial potential, and apoptosis in sample C-treated MCF-7 cells. A qPCR array of sample C-treated MCF-7 cells showed crosstalk between different pathways of apoptosis, especially BIRC5, BCL2L2, and TNFRSF1A genes. Immunofluorescence analysis affirmed the localization of p53, bax, bcl2, MAPKPK2, PARP-1, and caspase-3 proteins in exposed cells. Bioassay-guided fractionation of sample C revealed Neviotin A as the most active compound triggering maximum cell death in MCF-7, indicating its pharmacological potency for the development of a drug for the treatment of human breast cancer.