宫颈癌是全球卫生问题研究中的重要课题，在全球女性癌症病例中排名第四。它是中国专注于预防和治疗的九种重点癌症之一，也是唯一可以通过疫苗预防的癌症。系统有效的人乳头状瘤病毒（HPV）感染筛查可以降低宫颈癌发病率和死亡率。本文设计了一种电化学传感器，采用双信号放大检测HPV 16。改进了APTES修饰的玻碳电极以提高稳定性。同时，采用了金纳米粒子和链式放大反应进行信号放大。该电化学传感器的检测限为1.731×10-16 mol/L，目标检测范围的线性响应为1.0×10-13 mol/L至1.0×10-5 mol/L（R2 = 0.99232）。血清样品回收测试显示其具有良好的抗干扰性，与团队先前的研究相比，在各个方面的性能都得到了不同程度的改善。设计的传感器以低成本、高灵敏度和稳定性为原则，为宫颈癌预防和电化学生物传感器的未来发展提供了新的思路。

Cervical cancer is an important topic in the study of global health issues, ranking fourth among women's cancer cases in the world. It is one of the nine major cancers that China is focusing on preventing and treating, and it is the only cancer that can be prevented through vaccination. Systematic and effective screening for human papilloma (HPV) infection, which is closely linked to the development of cervical cancer, can reduce cervical cancer incidence and mortality. In this paper, an electrochemical sensor was designed to detect HPV 16 using dual-signal amplification. An APTES-modified glassy carbon electrode was used for improved stability. Gold nanoparticles and a chain amplification reaction were combined for signal amplification. The limit of detection (LOD) of this electrochemical sensor was 1.731 × 10-16 mol/L, and the linear response of the target detector range was from 1.0 × 10-13 mol/L to 1.0 × 10-5 mol/L (R2 = 0.99232). The test of serum sample recovery showed that it has good anti-interference, and the performance of all aspects was improved to different degrees compared with the previous research from the team. The designed sensor is centered around the principles of low cost, high sensitivity and stability, which provides new ideas for the future development of cervical cancer prevention and electrochemical biosensors.