纳米带生物传感器用于检测前列腺癌微小RNA标志物。
Nanoribbon Biosensor-Based Detection of microRNA Markers of Prostate Cancer.
发表日期：2023 Aug 30
作者：
Yuri D Ivanov, Kristina A Malsagova, Kristina V Goldaeva, Svetlana I Kapustina, Tatyana O Pleshakova, Vladimir P Popov, Andrey F Kozlov, Rafael A Galiullin, Ivan D Shumov, Dmitry V Enikeev, Natalia V Potoldykova, Vadim S Ziborov, Oleg F Petrov, Alexander Y Dolgoborodov, Alexander V Glukhov, Sergey V Novikov, Victoria K Grabezhova, Evgeniy S Yushkov, Vladimir A Konev, Oleg B Kovalev, Alexander I Archakov
来源：
BIOSENSORS & BIOELECTRONICS
摘要：
前列腺癌（PC）是老年男性死亡的主要原因之一。由于早期无症状，PC往往在较晚的进展阶段被诊断出来。因此，及早发现PC对有效的治疗至关重要。本研究旨在同时高灵敏度地检测人类血浆样品中一系列与PC相关的微小RNA（miRNA）。为实现这一目标，采用了基于“硅-绝缘体”结构（SOI-NR生物传感器）的纳带生物传感器系统。为了实现生物特异性对目标miRNA的检测，将个别纳带的表面敏化为与目标miRNA互补的DNA寡核苷酸探针（oDNA探针）。我们的生物传感器可检测到的核酸最低浓度为1.1 × 10-17 M。同时也证明了成功检测到来自PC患者真实血浆样品中的目标miRNA。我们相信，开发基于纳米技术的高灵敏度生物传感器用于检测PC标志物是朝向个体化医学的一步。
Prostate cancer (PC) is one of the major causes of death among elderly men. PC is often diagnosed later in progression due to asymptomatic early stages. Early detection of PC is thus crucial for effective PC treatment. The aim of this study is the simultaneous highly sensitive detection of a palette of PC-associated microRNAs (miRNAs) in human plasma samples. With this aim, a nanoribbon biosensor system based on "silicon-on-insulator" structures (SOI-NR biosensor) has been employed. In order to provide biospecific detection of the target miRNAs, the surface of individual nanoribbons has been sensitized with DNA oligonucleotide probes (oDNA probes) complementary to the target miRNAs. The lowest concentration of nucleic acids, detectable with our biosensor, has been found to be 1.1 × 10-17 M. The successful detection of target miRNAs, isolated from real plasma samples of PC patients, has also been demonstrated. We believe that the development of highly sensitive nanotechnology-based biosensors for the detection of PC markers is a step towards personalized medicine.