研究动态
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KSCBi005-A-8(hiPSC-PD-L1KO)是一种PD-L1敲除的人诱导多能干细胞系,用于展示PD-1/PD-L1轴的作用。

KSCBi005-A-8(hiPSC-PD-L1KO), a PD-L1 knockout human induced pluripotent stem cell line for demonstrating the role of the PD-1/PD-L1 axis.

发表日期:2023 Sep 01
作者: Hyeong-Jun Han, Jung-Hyun Kim
来源: Stem Cell Research & Therapy

摘要:

程序化细胞死亡配体1 (PD-L1) 是一种跨膜蛋白,用于减弱宿主免疫应答对肿瘤细胞的作用。癌细胞表面的PD-L1配体与T细胞上的PD-1跨膜受体结合,抑制了T细胞的功能。利用CRISPR-Cas9基因组编辑技术,我们在人诱导多能干细胞(hiPSCs)中生成了双等位基因的PD-L1突变体。PD-L1纯合突变的hiPSCs保留了其正常的形态、基因表达和体内分化潜力。版权所有 © 2023 作者。由Elsevier B.V.出版。保留所有权利。
Programmed Cell Death Ligand 1 (PD-L1) is a trans-membrane protein that attenuates the host immune response to tumor cells. PD-L1 ligand on the surface of the cancer binds to PD-1 transmembrane receptors on T cells and exhausts T cells function. Using CRISPR-Cas9 genome editing, we generated biallelic PD-L1 mutants in human induced pluripotent stem cells (hiPSCs). The PD-L1 homozygous-knockout hiPSCs retained their normal morphology, gene expression, and in vivo differentiation potential.Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.