聚合物封装金属纳米颗粒的合成是一个不断发展的领域，由于其在新技术开发中的长期应用。本研究描述了使用山奈酚合成壳聚糖/银纳米复合材料，用于抗癌和杀菌活性。通过出现棕色颜色和438nm附近的紫外吸收，确认了Kf-CS/Ag纳米复合材料的形成。利用红外研究确定合成纳米复合材料中存在Kf和CS。透射电子显微镜分析表明，合成的纳米复合材料具有主要均匀的球形形状，尺寸范围为7-10nm。能谱分析显示纳米复合材料中存在Ag、C和N元素。此外，Kf-CS/Ag纳米复合材料在体外抑制三阴性乳腺癌（TNBC）细胞的能力被证明，其IC50值为53μg/mL。此外，DAPI和AO/EtBr等荧光实验证实了Kf-CS/Ag纳米复合材料诱导MDA-MB-231细胞凋亡的能力。合成的Kf-CS/Ag纳米复合材料对金黄色葡萄球菌和铜绿假单胞菌表现出显著且剂量依赖的杀菌活性。因此，所得到的结果表明，合成的纳米复合材料可以作为生物灭菌纳米复合材料在生物医学领域中潜在应用于改善人类健康。版权所有 © 2023. 由Elsevier Inc.出版。

The synthesis of polymer-encapsulated metal nanoparticles is a growing field of area due to their long-term uses in the development of new technologies. The present study describes the synthesis of chitosan/silver nanocomposite using kaempferol for anticancer and bactericidal activity. The formation of Kf-CS/Ag nanocomposite was confirmed by the development of a brown color and UV-absorbance around 438 nm. The IR study was utilized to determine the existence of Kf and CS in the synthesized nanocomposite. TEM analysis demonstrated that the synthesized nanocomposite have a predominantly uniform spherical shape and size ranges 7-10 nm. EDX spectrum showed the existence of Ag, C, and N elements in the nanocomposite material. Further, Kf-CS/Ag nanocomposite exhibited potential in vitro inhibitory property against triple-negative breast cancer (TNBC) cells and their IC50 values was found to be 53 μg/mL. Moreover, fluorescent assays such as DAPI and AO/EtBr confirmed the apoptosis induction ability of Kf-CS/Ag nanocomposite in MDA-MB-231 cells. The synthesized Kf-CS/Ag nanocomposite showed significant and dose-depended bactericidal property against S. aureus and P. aeruginosa. Thus, the obtained findings demonstrated that the synthesized nanocomposite can be potentially used to improve human health as biocidal nanocomposite in biomedical sectors.Copyright © 2023. Published by Elsevier Inc.